Gene description for Sae1
Gene name SUMO1 activating enzyme subunit 1
Gene symbol Sae1
Other names/aliases 2400010M20Rik
2610044L12Rik
AL033372
AOS1
AW743391
D7Ertd177e
HSPC140
Sua1
Uble1a
Species Mus musculus
 Database cross references - Sae1
ExoCarta ExoCarta_56459
Vesiclepedia VP_56459
Entrez Gene 56459
UniProt Q9R1T2  
 Sae1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sae1
Molecular Function
    SUMO activating enzyme activity GO:0019948 IBA
    SUMO activating enzyme activity GO:0019948 ISO
    SUMO activating enzyme activity GO:0019948 ISS
    ATP-dependent protein binding GO:0043008 IEA
    ATP-dependent protein binding GO:0043008 ISO
    small protein activating enzyme binding GO:0044388 IEA
    small protein activating enzyme binding GO:0044388 ISO
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
Biological Process
    protein sumoylation GO:0016925 IBA
    protein sumoylation GO:0016925 IEA
    protein sumoylation GO:0016925 ISO
    protein sumoylation GO:0016925 ISS
    positive regulation of protein sumoylation GO:0033235 IEA
    positive regulation of protein sumoylation GO:0033235 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    SUMO activating enzyme complex GO:0031510 IBA
    SUMO activating enzyme complex GO:0031510 IDA
    SUMO activating enzyme complex GO:0031510 ISO
    SUMO activating enzyme complex GO:0031510 ISS
 Experiment description of studies that identified Sae1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sae1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Faf2  
Co-fractionation Mus musculus
2 Aars 234734
Co-fractionation Mus musculus
3 USP2  
Biochemical Activity Homo sapiens
4 Ppp2r5e 26932
Co-fractionation Mus musculus
5 Uba2 50995
Co-fractionation Mus musculus
6 Tpd52l2  
Co-fractionation Mus musculus
7 Osgep  
Co-fractionation Mus musculus
8 Pank4  
Co-fractionation Mus musculus
9 Ube2e3  
Co-fractionation Mus musculus
10 Dhps  
Co-fractionation Mus musculus
11 Pde12  
Co-fractionation Mus musculus
12 Wdr4  
Co-fractionation Mus musculus
13 Dpp9  
Co-fractionation Mus musculus
14 Camk1  
Co-fractionation Mus musculus
15 Sephs1  
Co-fractionation Mus musculus
16 Calu  
Co-fractionation Mus musculus
17 Lsm1  
Co-fractionation Mus musculus
18 Rprd1b  
Co-fractionation Mus musculus
19 Rabggtb  
Co-fractionation Mus musculus
20 Hmox2  
Co-fractionation Mus musculus
21 Fancd2  
Affinity Capture-MS Mus musculus
22 Krt19 16669
Co-fractionation Mus musculus
23 Ube2i  
Co-fractionation Mus musculus
24 Ppa1 67895
Co-fractionation Mus musculus
25 Cul5 75717
Co-fractionation Mus musculus
View the network image/svg+xml



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