Gene description for RAB20
Gene name RAB20, member RAS oncogene family
Gene symbol RAB20
Other names/aliases -
Species Homo sapiens
 Database cross references - RAB20
ExoCarta ExoCarta_55647
Vesiclepedia VP_55647
Entrez Gene 55647
HGNC 18260
UniProt Q9NX57  
 RAB20 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
 Gene ontology annotations for RAB20
Molecular Function
    GTPase activity GO:0003924 IBA
    GTP binding GO:0005525 IEA
Biological Process
    intracellular protein transport GO:0006886 IBA
    endocytosis GO:0006897 IBA
    cellular response to type II interferon GO:0071346 IEA
    phagosome acidification GO:0090383 IMP
    phagosome-lysosome fusion GO:0090385 IMP
Subcellular Localization
    early endosome GO:0005769 IBA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IBA
    endomembrane system GO:0012505 IBA
    endocytic vesicle GO:0030139 IBA
    phagocytic vesicle membrane GO:0030670 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    phagocytic vesicle GO:0045335 IDA
 Experiment description of studies that identified RAB20 in sEVs
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for RAB20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBP1 7342
Affinity Capture-MS Homo sapiens
2 NXF1 10482
Affinity Capture-RNA Homo sapiens
3 MAGEB10  
Affinity Capture-MS Homo sapiens
4 CTU2 348180
Affinity Capture-MS Homo sapiens
5 TFCP2 7024
Affinity Capture-MS Homo sapiens
6 ANKRD13D  
Affinity Capture-MS Homo sapiens
7 RAP1GDS1 5910
Affinity Capture-MS Homo sapiens
8 SPATA20  
Affinity Capture-MS Homo sapiens
9 ALKBH1  
Affinity Capture-MS Homo sapiens
10 CHM 1121
Affinity Capture-MS Homo sapiens
11 INF2 64423
Affinity Capture-MS Homo sapiens
12 PGGT1B 5229
Affinity Capture-MS Homo sapiens
13 BCL10  
Affinity Capture-MS Homo sapiens
14 RABGGTA 5875
Affinity Capture-MS Homo sapiens
15 CHML  
Affinity Capture-MS Homo sapiens
16 DDX58 23586
Affinity Capture-RNA Homo sapiens
17 MINA 84864
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RAB20 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Post-translational protein modification TAS Reactome
RAB geranylgeranylation TAS Reactome





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