Gene description for Prdx4
Gene name peroxiredoxin 4
Gene symbol Prdx4
Other names/aliases AOE372
Prx-iv
Prx4
TRANK
Species Mus musculus
 Database cross references - Prdx4
ExoCarta ExoCarta_53381
Vesiclepedia VP_53381
Entrez Gene 53381
UniProt O08807  
 Prdx4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prdx4
Molecular Function
    protein binding GO:0005515 IPI
    thioredoxin peroxidase activity GO:0008379 IBA
    identical protein binding GO:0042802 IPI
    molecular adaptor activity GO:0060090 ISO
    molecular sequestering activity GO:0140313 IEA
    molecular sequestering activity GO:0140313 ISO
    thioredoxin-dependent peroxiredoxin activity GO:0140824 IEA
Biological Process
    response to oxidative stress GO:0006979 IBA
    response to oxidative stress GO:0006979 IMP
    spermatogenesis GO:0007283 IMP
    male gonad development GO:0008584 IMP
    peptidyl-proline hydroxylation to 4-hydroxy-L-proline GO:0018401 IGI
    protein maturation by protein folding GO:0022417 IGI
    extracellular matrix organization GO:0030198 IGI
    hydrogen peroxide catabolic process GO:0042744 IBA
    cell redox homeostasis GO:0045454 IBA
    cell redox homeostasis GO:0045454 IGI
    reactive oxygen species metabolic process GO:0072593 IDA
    cellular oxidant detoxification GO:0098869 IEA
    negative regulation of male germ cell proliferation GO:2000255 IMP
Subcellular Localization
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    smooth endoplasmic reticulum GO:0005790 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified Prdx4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prdx4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Txndc5 105245
Co-fractionation Mus musculus
2 Atxn1  
Proximity Label-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Tln1 21894
Co-fractionation Mus musculus
5 Usp7  
Affinity Capture-MS Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Prdx4 is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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