Gene description for ACHE
Gene name acetylcholinesterase (Yt blood group)
Gene symbol ACHE
Other names/aliases ACEE
ARACHE
N-ACHE
YT
Species Homo sapiens
 Database cross references - ACHE
ExoCarta ExoCarta_43
Vesiclepedia VP_43
Entrez Gene 43
HGNC 108
MIM 100740
UniProt P22303  
 ACHE identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Cervical cancer cells 34282141    
Cervical cancer cells 34282141    
Cervical cancer cells 34282141    
Cervical cancer cells 34282141    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for ACHE
Molecular Function
    amyloid-beta binding GO:0001540 TAS
    acetylcholinesterase activity GO:0003990 IBA
    acetylcholinesterase activity GO:0003990 IDA
    acetylcholinesterase activity GO:0003990 IMP
    acetylcholinesterase activity GO:0003990 TAS
    cholinesterase activity GO:0004104 IDA
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IDA
    hydrolase activity GO:0016787 IDA
    serine hydrolase activity GO:0017171 IDA
    acetylcholine binding GO:0042166 IDA
    acetylcholine binding GO:0042166 NAS
    protein homodimerization activity GO:0042803 IPI
    laminin binding GO:0043236 IDA
Biological Process
    acetylcholine catabolic process in synaptic cleft GO:0001507 NAS
    regulation of receptor recycling GO:0001919 IEA
    osteoblast development GO:0002076 IEP
    acetylcholine catabolic process GO:0006581 IBA
    acetylcholine catabolic process GO:0006581 IDA
    acetylcholine catabolic process GO:0006581 TAS
    cell adhesion GO:0007155 IDA
    nervous system development GO:0007399 TAS
    synapse assembly GO:0007416 TAS
    receptor internalization GO:0031623 IEA
    negative regulation of synaptic transmission, cholinergic GO:0032223 IC
    amyloid precursor protein metabolic process GO:0042982 TAS
    positive regulation of protein secretion GO:0050714 TAS
    retina development in camera-type eye GO:0060041 IEA
    acetylcholine receptor signaling pathway GO:0095500 IEA
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 TAS
    basement membrane GO:0005604 NAS
    extracellular space GO:0005615 IEA
    nucleus GO:0005634 IEA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IEA
    membrane GO:0016020 IDA
    neuromuscular junction GO:0031594 IEA
    synaptic cleft GO:0043083 IEA
    synapse GO:0045202 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    side of membrane GO:0098552 IEA
 Experiment description of studies that identified ACHE in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1325
MISEV standards
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34282141    
Organism Homo sapiens
Experiment description Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
Authors "Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C."
Journal name Nat Commun
Publication year 2021
Sample Cervical cancer cells
Sample name HeLa - 200K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
6
Experiment ID 1328
MISEV standards
Biophysical techniques
✔
CD63|SDCBP|CD81|CD9
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34282141    
Organism Homo sapiens
Experiment description Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
Authors "Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C."
Journal name Nat Commun
Publication year 2021
Sample Cervical cancer cells
Sample name HeLa - 200K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
7
Experiment ID 1329
MISEV standards
Biophysical techniques
✔
CD63|SDCBP|LAMP1|CD81|CD9
Enriched markers
✔
CANX
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34282141    
Organism Homo sapiens
Experiment description Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
Authors "Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C."
Journal name Nat Commun
Publication year 2021
Sample Cervical cancer cells
Sample name HeLa - 200K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 1330
MISEV standards
Biophysical techniques
✔
CD63|SDCBP|CD81|CD9
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34282141    
Organism Homo sapiens
Experiment description Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
Authors "Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C."
Journal name Nat Commun
Publication year 2021
Sample Cervical cancer cells
Sample name HeLa - 200K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ACHE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
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