Gene description for AQP5
Gene name aquaporin 5
Gene symbol AQP5
Other names/aliases AQP-5
PPKB
Species Homo sapiens
 Database cross references - AQP5
ExoCarta ExoCarta_362
Vesiclepedia VP_362
Entrez Gene 362
HGNC 638
MIM 600442
UniProt P55064  
 AQP5 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Saliva 19199708    
 Gene ontology annotations for AQP5
Molecular Function
    protein binding GO:0005515 IPI
    water channel activity GO:0015250 IBA
    water channel activity GO:0015250 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    water transport GO:0006833 IBA
    water transport GO:0006833 IDA
    carbon dioxide transport GO:0015670 IBA
    carbon dioxide transport GO:0015670 IDA
    pancreatic juice secretion GO:0030157 IEP
    odontogenesis GO:0042476 IEP
    saliva secretion GO:0046541 IEA
    camera-type eye morphogenesis GO:0048593 IEA
    protein homotetramerization GO:0051289 IDA
    transmembrane transport GO:0055085 IEA
    cellular hypotonic response GO:0071476 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    microvillus GO:0005902 IEA
    basal plasma membrane GO:0009925 IEA
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    cytoplasmic vesicle membrane GO:0030659 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified AQP5 in sEVs
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for AQP5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPK 3190
Two-hybrid Homo sapiens
2 ATP1A2 477
Affinity Capture-MS Homo sapiens
3 CREB3  
Two-hybrid Homo sapiens
4 KRT40  
Two-hybrid Homo sapiens
5 NOTCH2NL 388677
Two-hybrid Homo sapiens
6 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
7 MDFI  
Two-hybrid Homo sapiens
8 KRT31 3881
Two-hybrid Homo sapiens
9 WNT2  
Two-hybrid Homo sapiens
10 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
11 GLRX 2745
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which AQP5 is involved
PathwayEvidenceSource
Aquaporin-mediated transport TAS Reactome
Passive transport by Aquaporins TAS Reactome
Transport of small molecules TAS Reactome





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