Gene description for Hist2h3b
Gene name histone cluster 2, H3b
Gene symbol Hist2h3b
Other names/aliases H3-615
H3-616
Species Mus musculus
 Database cross references - Hist2h3b
ExoCarta ExoCarta_319154
Vesiclepedia VP_319154
Entrez Gene 319154
UniProt P84228  
 Hist2h3b identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
Oligodendrocytes 21136642    
 Gene ontology annotations for Hist2h3b
Subcellular Localization
    nucleosome GO:0000786 ISO
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Hist2h3b in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Hist2h3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Affinity Capture-MS Mus musculus
2 Rpl27a 26451
Cross-Linking-MS (XL-MS) Mus musculus
3 Hist1h2bg  
Cross-Linking-MS (XL-MS) Mus musculus
4 Hist2h3b 319154
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml
 Pathways in which Hist2h3b is involved
PathwayEvidenceSource
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Chromatin modifying enzymes IEA Reactome
Chromatin organization IEA Reactome
Condensation of Prophase Chromosomes IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes IEA Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of gene expression IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-dependent gene expression IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
HATs acetylate histones IEA Reactome
HDACs deacetylate histones IEA Reactome
HDMs demethylate histones IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Interleukin-7 signaling IEA Reactome
M Phase IEA Reactome
Mitotic Prophase IEA Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis IEA Reactome
PKMTs methylate histone lysines IEA Reactome
PRC2 methylates histones and DNA IEA Reactome
Regulation of endogenous retroelements IEA Reactome
Regulation of endogenous retroelements by KRAB-ZFP proteins IEA Reactome
RMTs methylate histone arginines IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function IEA Reactome
Signal Transduction IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Transcriptional regulation by RUNX1 IEA Reactome





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