Gene description for Pdia3
Gene name protein disulfide isomerase family A, member 3
Gene symbol Pdia3
Other names/aliases ER60
ERp57
Grp58
Species Rattus norvegicus
 Database cross references - Pdia3
ExoCarta ExoCarta_29468
Vesiclepedia VP_29468
Entrez Gene 29468
UniProt P11598  
 Pdia3 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Pdia3
Molecular Function
    protein disulfide isomerase activity GO:0003756 IBA
    protein disulfide isomerase activity GO:0003756 IEA
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IDA
    protein-disulfide reductase activity GO:0015035 IEA
    protein-disulfide reductase activity GO:0015035 ISO
    protein-disulfide reductase (glutathione) activity GO:0019153 IMP
    MHC class I protein binding GO:0042288 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    response to hypoxia GO:0001666 IEP
    adaptive immune response GO:0002250 IEA
    peptide antigen assembly with MHC class I protein complex GO:0002502 IEA
    peptide antigen assembly with MHC class I protein complex GO:0002502 ISO
    response to ischemia GO:0002931 IEP
    protein folding GO:0006457 IBA
    circadian rhythm GO:0007623 IEP
    response to nutrient levels GO:0031667 IEP
    response to genistein GO:0033595 IEP
    response to endoplasmic reticulum stress GO:0034976 IBA
    response to leptin GO:0044321 IEP
    response to ethanol GO:0045471 IEP
    response to hyperoxia GO:0055093 IEP
    platelet aggregation GO:0070527 IEA
    platelet aggregation GO:0070527 ISO
    cellular response to vitamin D GO:0071305 IDA
    cellular response to transforming growth factor beta stimulus GO:0071560 IEP
    extrinsic apoptotic signaling pathway GO:0097191 IEA
    extrinsic apoptotic signaling pathway GO:0097191 ISO
    cellular response to interleukin-7 GO:0098761 IEA
    cellular response to interleukin-7 GO:0098761 ISO
    response to benzene GO:1901423 IEP
    positive regulation of protein folding GO:1903334 IDA
    cellular response to nonylphenol GO:1904148 IEP
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IEA
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 ISO
Subcellular Localization
    acrosomal vesicle GO:0001669 IDA
    extracellular region GO:0005576 IDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum lumen GO:0005788 IEA
    smooth endoplasmic reticulum GO:0005790 IDA
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    apical plasma membrane GO:0016324 IDA
    melanosome GO:0042470 IEA
    MHC class I peptide loading complex GO:0042824 IDA
    MHC class I peptide loading complex GO:0042824 ISO
    TAP complex GO:0042825 IDA
    Tapasin-ERp57 complex GO:0061779 IEA
    Tapasin-ERp57 complex GO:0061779 ISO
 Experiment description of studies that identified Pdia3 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pdia3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Stat3  
Affinity Capture-MS Rattus norvegicus
2 Slc12a3  
Affinity Capture-Western Rattus norvegicus
3 RARA 5914
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
4 Gja1  
Affinity Capture-MS Rattus norvegicus
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