Gene description for Ehd2
Gene name EH-domain containing 2
Gene symbol Ehd2
Other names/aliases BC027084
C130052H20Rik
Species Mus musculus
 Database cross references - Ehd2
ExoCarta ExoCarta_259300
Vesiclepedia VP_259300
Entrez Gene 259300
UniProt Q8BH64  
 Ehd2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ehd2
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    GTP binding GO:0005525 IEA
    hydrolase activity GO:0016787 IEA
    protein domain specific binding GO:0019904 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    endocytosis GO:0006897 IBA
    endocytosis GO:0006897 IDA
    cortical actin cytoskeleton organization GO:0030866 IDA
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISS
    cilium assembly GO:0060271 IBA
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IDA
    plasma membrane tubulation GO:0097320 IDA
    positive regulation of myoblast fusion GO:1901741 IMP
    positive regulation of endocytic recycling GO:2001137 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    early endosome GO:0005769 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    caveola GO:0005901 IDA
    caveola GO:0005901 ISO
    microtubule cytoskeleton GO:0015630 IEA
    microtubule cytoskeleton GO:0015630 ISO
    membrane GO:0016020 IDA
    endocytic vesicle GO:0030139 IBA
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    perinuclear region of cytoplasm GO:0048471 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
    recycling endosome membrane GO:0055038 IBA
    recycling endosome membrane GO:0055038 ISO
    recycling endosome membrane GO:0055038 ISS
 Experiment description of studies that identified Ehd2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ehd2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ehd2 259300
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ehd2 is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production IEA Reactome
Hemostasis IEA Reactome





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