Gene description for ZNF473
Gene name
zinc finger protein 473
Gene symbol
ZNF473
Other names/aliases
HZFP100 ZN473
Species
Homo sapiens
Database cross references - ZNF473
ExoCarta
ExoCarta_25888
Entrez Gene
25888
HGNC
23239
UniProt
Q8WTR7
ZNF473 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells
26054723
Hepatocellular carcinoma cells
26054723
Gene ontology annotations for ZNF473
Experiment description of studies that identified ZNF473 in exosomes
1
Experiment ID
235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
2
Experiment ID
236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
Protein-protein interactions for ZNF473
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
SLBP
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
2
TRIM41
90933
Two-hybrid
Homo sapiens
3
LSM11
Reconstituted Complex
Homo sapiens
4
ZNF202
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which ZNF473 is involved