Gene description for CFAP45
Gene name cilia and flagella associated protein 45
Gene symbol CFAP45
Other names/aliases CCDC19
NESG1
Species Homo sapiens
 Database cross references - CFAP45
ExoCarta ExoCarta_25790
Vesiclepedia VP_25790
Entrez Gene 25790
HGNC 17229
MIM 605152
UniProt Q9UL16  
 CFAP45 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CFAP45
Molecular Function
    protein binding GO:0005515 IPI
    AMP binding GO:0016208 IDA
Biological Process
    flagellated sperm motility GO:0030317 IMP
    flagellated sperm motility GO:0030317 ISS
    epithelial cilium movement involved in determination of left/right asymmetry GO:0060287 IEA
    regulation of cilium beat frequency involved in ciliary motility GO:0060296 IEA
    establishment of left/right asymmetry GO:0061966 IMP
    cerebrospinal fluid circulation GO:0090660 IEA
Subcellular Localization
    extracellular region GO:0005576 IEA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    axonemal microtubule GO:0005879 IDA
    axonemal microtubule GO:0005879 ISS
    axoneme GO:0005930 IDA
    sperm flagellum GO:0036126 ISS
    9+0 motile cilium GO:0097728 IEA
    9+2 motile cilium GO:0097729 IDA
    axonemal B tubule inner sheath GO:0160112 ISS
 Experiment description of studies that identified CFAP45 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CFAP45
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 KRT8 3856
Cross-Linking-MS (XL-MS) Homo sapiens
3 MECP2 4204
Affinity Capture-MS Homo sapiens
4 TRIO 7204
Co-fractionation Homo sapiens
5 OBSL1 23363
Affinity Capture-MS Homo sapiens
6 GLS 2744
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
8 ENKUR  
Two-hybrid Homo sapiens
9 SAR1B 51128
Affinity Capture-MS Homo sapiens
10 H2AFX 3014
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CFAP45 is involved
No pathways found





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