Gene description for Ywhaz
Gene name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
Gene symbol Ywhaz
Other names/aliases 14-3-3z
Species Rattus norvegicus
 Database cross references - Ywhaz
ExoCarta ExoCarta_25578
Vesiclepedia VP_25578
Entrez Gene 25578
UniProt P63102  
 Ywhaz identified in sEVs derived from the following tissue/cell type
Cortical neurones 16446100    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Ywhaz
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    protein domain specific binding GO:0019904 ISS
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    transmembrane transporter binding GO:0044325 IEA
    transmembrane transporter binding GO:0044325 ISO
    protein-containing complex binding GO:0044877 IPI
    phosphoserine residue binding GO:0050815 IEA
    phosphoserine residue binding GO:0050815 ISO
    phosphoserine residue binding GO:0050815 ISS
    DNA-binding transcription factor binding GO:0140297 IEA
    DNA-binding transcription factor binding GO:0140297 ISO
    protein sequestering activity GO:0140311 IEA
    protein sequestering activity GO:0140311 ISO
    protein sequestering activity GO:0140311 ISS
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    angiogenesis GO:0001525 IEA
    angiogenesis GO:0001525 ISO
    angiogenesis GO:0001525 ISO
    histamine secretion by mast cell GO:0002553 IMP
    respiratory system process GO:0003016 IEA
    respiratory system process GO:0003016 ISO
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    protein targeting GO:0006605 IEA
    protein targeting GO:0006605 ISO
    protein targeting to mitochondrion GO:0006626 IDA
    signal transduction GO:0007165 IBA
    signal transduction GO:0007165 ISO
    signal transduction GO:0007165 ISS
    synaptic target recognition GO:0008039 IEA
    synaptic target recognition GO:0008039 ISO
    protein localization GO:0008104 IBA
    response to xenobiotic stimulus GO:0009410 IDA
    lung development GO:0030324 IEA
    lung development GO:0030324 ISO
    regulation of protein stability GO:0031647 ISO
    tube formation GO:0035148 IEA
    tube formation GO:0035148 ISO
    tube formation GO:0035148 ISO
    cellular response to glucose starvation GO:0042149 IEA
    cellular response to glucose starvation GO:0042149 ISO
    regulation of programmed cell death GO:0043067 IEA
    regulation of programmed cell death GO:0043067 ISO
    negative regulation of innate immune response GO:0045824 IEA
    negative regulation of innate immune response GO:0045824 ISO
    establishment of Golgi localization GO:0051683 IEA
    establishment of Golgi localization GO:0051683 ISO
    ERK1 and ERK2 cascade GO:0070371 IEA
    ERK1 and ERK2 cascade GO:0070371 ISO
    regulation of ERK1 and ERK2 cascade GO:0070372 IEA
    regulation of ERK1 and ERK2 cascade GO:0070372 ISO
    regulation of ERK1 and ERK2 cascade GO:0070372 ISS
    regulation of synapse maturation GO:0090128 IEA
    regulation of synapse maturation GO:0090128 ISO
    Golgi reassembly GO:0090168 IEA
    Golgi reassembly GO:0090168 ISO
    negative regulation of protein localization to nucleus GO:1900181 IEA
    negative regulation of protein localization to nucleus GO:1900181 ISO
    negative regulation of TORC1 signaling GO:1904262 IEA
    negative regulation of TORC1 signaling GO:1904262 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cell leading edge GO:0031252 IDA
    protein-containing complex GO:0032991 IDA
    melanosome GO:0042470 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IEA
    hippocampal mossy fiber to CA3 synapse GO:0098686 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
    postsynaptic specialization GO:0099572 IDA
    postsynaptic specialization GO:0099572 IMP
 Experiment description of studies that identified Ywhaz in sEVs
1
Experiment ID 30
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors "Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R"
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
10
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
11
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
12
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ywhaz
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gja1  
Affinity Capture-MS Rattus norvegicus
2 Sik3  
Affinity Capture-Western Rattus norvegicus
3 Map3k3  
Two-hybrid Rattus norvegicus
4 MAPT  
Reconstituted Complex Homo sapiens
5 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
6 Clic4 83718
Affinity Capture-MS Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Reconstituted Complex Rattus norvegicus
7 Park7 117287
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Ywhaz is involved
PathwayEvidenceSource
Activation of BAD and translocation to mitochondria IEA Reactome
Activation of BH3-only proteins IEA Reactome
Adaptive Immune System IEA Reactome
Apoptosis IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Deactivation of the beta-catenin transactivating complex IEA Reactome
FOXO-mediated transcription IEA Reactome
G2/M Checkpoints IEA Reactome
G2/M DNA damage checkpoint IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GP1b-IX-V activation signalling IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling IEA Reactome
Intrinsic Pathway for Apoptosis IEA Reactome
KSRP (KHSRP) binds and destabilizes mRNA IEA Reactome
Metabolism of RNA IEA Reactome
NOTCH4 Activation and Transmission of Signal to the Nucleus IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Programmed Cell Death IEA Reactome
Rap1 signalling IEA Reactome
Regulation of localization of FOXO transcription factors IEA Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases activate PKNs IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Signal Transduction IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by NOTCH IEA Reactome
Signaling by NOTCH4 IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by WNT IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
TP53 Regulates Metabolic Genes IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome





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