Gene description for Hspb1
Gene name heat shock protein B1
Gene symbol Hspb1
Other names/aliases Hsp25
Hsp27
Species Rattus norvegicus
 Database cross references - Hspb1
ExoCarta ExoCarta_24471
Vesiclepedia VP_24471
Entrez Gene 24471
 Hspb1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Hspb1
Molecular Function
    protein kinase C binding GO:0005080 IEA
    protein kinase C binding GO:0005080 ISO
    protein binding GO:0005515 IPI
    protein kinase C inhibitor activity GO:0008426 IEA
    protein kinase C inhibitor activity GO:0008426 ISO
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 ISO
    identical protein binding GO:0042802 ISS
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    ubiquitin binding GO:0043130 IDA
    protein folding chaperone GO:0044183 IEA
    protein folding chaperone GO:0044183 ISO
    protein folding chaperone GO:0044183 ISS
    unfolded protein binding GO:0051082 IBA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
Biological Process
    regulation of protein phosphorylation GO:0001932 ISO
    regulation of protein phosphorylation GO:0001932 ISS
    response to ischemia GO:0002931 IEP
    negative regulation of protein kinase activity GO:0006469 ISO
    female pregnancy GO:0007565 IEP
    response to heat GO:0009408 IBA
    response to virus GO:0009615 IEA
    response to virus GO:0009615 ISO
    positive regulation of neuron projection development GO:0010976 IGI
    positive regulation of interleukin-1 beta production GO:0032731 IEA
    positive regulation of interleukin-1 beta production GO:0032731 ISO
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    intracellular signal transduction GO:0035556 IEA
    intracellular signal transduction GO:0035556 ISO
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IEA
    cellular response to vascular endothelial growth factor stimulus GO:0035924 ISO
    response to muscle stretch GO:0035994 IEP
    protein refolding GO:0042026 IBA
    negative regulation of apoptotic process GO:0043066 IBA
    negative regulation of apoptotic process GO:0043066 TAS
    regulation of canonical NF-kappaB signal transduction GO:0043122 IEA
    regulation of canonical NF-kappaB signal transduction GO:0043122 ISO
    positive regulation of blood vessel endothelial cell migration GO:0043536 IEA
    positive regulation of blood vessel endothelial cell migration GO:0043536 ISO
    negative regulation of regulatory T cell differentiation GO:0045590 IMP
    positive regulation of angiogenesis GO:0045766 IEA
    positive regulation of angiogenesis GO:0045766 ISO
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 ISO
    chaperone-mediated protein folding GO:0061077 IEA
    chaperone-mediated protein folding GO:0061077 ISO
    chaperone-mediated protein folding GO:0061077 ISS
    cellular response to hydrogen peroxide GO:0070301 IEP
    platelet aggregation GO:0070527 ISO
    cellular response to interleukin-11 GO:0071348 IEP
    anterograde axonal protein transport GO:0099641 IEA
    anterograde axonal protein transport GO:0099641 ISO
    anterograde axonal protein transport GO:0099641 ISS
    negative regulation of cellular response to oxidative stress GO:1900408 IMP
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 IEA
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 ISO
    cellular response to butyrate GO:1903545 IEP
    response to angiotensin GO:1990776 IEP
    positive regulation of endothelial cell chemotaxis GO:2001028 IEA
    positive regulation of endothelial cell chemotaxis GO:2001028 ISO
    negative regulation of apoptotic signaling pathway GO:2001234 ISO
Subcellular Localization
    proteasome complex GO:0000502 IDA
    cornified envelope GO:0001533 IEA
    cornified envelope GO:0001533 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    spindle GO:0005819 IEA
    plasma membrane GO:0005886 ISO
    Z disc GO:0030018 IEA
    Z disc GO:0030018 ISO
    axon GO:0030424 IDA
    dendrite GO:0030425 IDA
    M band GO:0031430 IDA
    I band GO:0031674 IDA
    perikaryon GO:0043204 IDA
    contractile muscle fiber GO:0043292 ISO
    cardiac myofibril GO:0097512 IDA
    postsynaptic density membrane GO:0098839 IDA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified Hspb1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hspb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cryab  
Two-hybrid Rattus norvegicus
2 Mapkapk5  
Affinity Capture-Western Rattus norvegicus
3 Hspbap1  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Two-hybrid Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
4 Hspb1 24471
Two-hybrid Rattus norvegicus
Two-hybrid Rattus norvegicus
5 Ubc  
Two-hybrid Rattus norvegicus
View the network image/svg+xml
 Pathways in which Hspb1 is involved
No pathways found





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