Gene description for Snrpe
Gene name small nuclear ribonucleoprotein E
Gene symbol Snrpe
Other names/aliases AL022645
C76690
SME
Species Mus musculus
 Database cross references - Snrpe
ExoCarta ExoCarta_20643
Vesiclepedia VP_20643
Entrez Gene 20643
UniProt P62305  
 Snrpe identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Snrpe
Molecular Function
    RNA binding GO:0003723 IEA
    U1 snRNP binding GO:1990446 ISO
Biological Process
    spliceosomal snRNP assembly GO:0000387 IBA
    spliceosomal snRNP assembly GO:0000387 ISO
    spliceosomal snRNP assembly GO:0000387 ISS
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    spliceosomal complex GO:0005681 ISO
    U5 snRNP GO:0005682 IBA
    U7 snRNP GO:0005683 ISO
    U7 snRNP GO:0005683 ISS
    U2-type spliceosomal complex GO:0005684 ISO
    U2-type spliceosomal complex GO:0005684 ISS
    U1 snRNP GO:0005685 IBA
    U1 snRNP GO:0005685 ISO
    U1 snRNP GO:0005685 ISS
    U2 snRNP GO:0005686 IBA
    U4 snRNP GO:0005687 IBA
    U4 snRNP GO:0005687 ISO
    U4 snRNP GO:0005687 ISS
    U12-type spliceosomal complex GO:0005689 IEA
    U12-type spliceosomal complex GO:0005689 ISO
    telomerase holoenzyme complex GO:0005697 IEA
    telomerase holoenzyme complex GO:0005697 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    methylosome GO:0034709 ISO
    methylosome GO:0034709 ISS
    pICln-Sm protein complex GO:0034715 IBA
    pICln-Sm protein complex GO:0034715 ISO
    pICln-Sm protein complex GO:0034715 ISS
    SMN-Sm protein complex GO:0034719 ISO
    SMN-Sm protein complex GO:0034719 ISS
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISS
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    U2-type catalytic step 2 spliceosome GO:0071007 ISO
    U2-type catalytic step 2 spliceosome GO:0071007 ISS
    precatalytic spliceosome GO:0071011 IBA
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Snrpe in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Snrpe
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3b1 81898
Co-fractionation Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Frg1  
Co-fractionation Mus musculus
4 U2af2 22185
Co-fractionation Mus musculus
5 Snrpd2 107686
Co-fractionation Mus musculus
6 Snrpd1 20641
Co-fractionation Mus musculus
7 Cul4b  
Co-fractionation Mus musculus
8 Unk  
Affinity Capture-RNA Mus musculus
9 Snrpg 68011
Co-fractionation Mus musculus
10 Fancd2  
Affinity Capture-MS Mus musculus
11 Plrg1  
Co-fractionation Mus musculus
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