Gene description for Slc12a2
Gene name solute carrier family 12, member 2
Gene symbol Slc12a2
Other names/aliases 9330166H04Rik
Nkcc1
mBSC2
sy-ns
Species Mus musculus
 Database cross references - Slc12a2
ExoCarta ExoCarta_20496
Vesiclepedia VP_20496
Entrez Gene 20496
UniProt P55012  
 Slc12a2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc12a2
Molecular Function
    protein binding GO:0005515 IPI
    sodium:potassium:chloride symporter activity GO:0008511 IBA
    sodium:potassium:chloride symporter activity GO:0008511 IC
    sodium:potassium:chloride symporter activity GO:0008511 ISO
    ammonium channel activity GO:0008519 IBA
    ammonium channel activity GO:0008519 ISO
    potassium ion transmembrane transporter activity GO:0015079 IMP
    chloride:monoatomic cation symporter activity GO:0015377 ISO
    protein kinase binding GO:0019901 ISO
    metal ion binding GO:0046872 IEA
    metal ion transmembrane transporter activity GO:0046873 ISO
    protein-folding chaperone binding GO:0051087 ISO
    Hsp90 protein binding GO:0051879 ISO
Biological Process
    potassium ion transport GO:0006813 ISO
    sodium ion transport GO:0006814 ISO
    chloride transport GO:0006821 ISO
    intracellular sodium ion homeostasis GO:0006883 IMP
    cell volume homeostasis GO:0006884 IBA
    cell volume homeostasis GO:0006884 IMP
    hyperosmotic response GO:0006972 ISO
    gamma-aminobutyric acid signaling pathway GO:0007214 ISO
    T cell chemotaxis GO:0010818 IMP
    T cell chemotaxis GO:0010818 ISO
    intracellular potassium ion homeostasis GO:0030007 IMP
    transepithelial chloride transport GO:0030321 ISO
    intracellular chloride ion homeostasis GO:0030644 IMP
    multicellular organism growth GO:0035264 IMP
    maintenance of blood-brain barrier GO:0035633 IMP
    sodium ion transmembrane transport GO:0035725 IBA
    cellular response to potassium ion GO:0035865 IMP
    positive regulation of cell volume GO:0045795 ISO
    detection of mechanical stimulus involved in sensory perception of sound GO:0050910 IMP
    chloride ion homeostasis GO:0055064 IBA
    potassium ion homeostasis GO:0055075 IBA
    sodium ion homeostasis GO:0055078 IBA
    branching involved in mammary gland duct morphogenesis GO:0060444 IMP
    mammary duct terminal end bud growth GO:0060763 IMP
    negative regulation of vascular wound healing GO:0061044 IMP
    transepithelial ammonium transport GO:0070634 ISO
    cellular response to lipopolysaccharide GO:0071222 IDA
    ammonium transmembrane transport GO:0072488 IBA
    ammonium transmembrane transport GO:0072488 ISO
    inorganic anion import across plasma membrane GO:0098658 IMP
    inorganic cation import across plasma membrane GO:0098659 ISO
    sodium ion import across plasma membrane GO:0098719 IMP
    regulation of spontaneous synaptic transmission GO:0150003 IGI
    regulation of spontaneous synaptic transmission GO:0150003 IMP
    regulation of spontaneous synaptic transmission GO:0150003 ISO
    chloride transmembrane transport GO:1902476 IBA
    chloride transmembrane transport GO:1902476 IMP
    positive regulation of aspartate secretion GO:1904450 IMP
    regulation of matrix metallopeptidase secretion GO:1904464 IMP
    potassium ion import across plasma membrane GO:1990573 IBA
    potassium ion import across plasma membrane GO:1990573 IMP
    cellular response to chemokine GO:1990869 IMP
    cellular response to chemokine GO:1990869 ISO
Subcellular Localization
    plasma membrane GO:0005886 ISO
    basal plasma membrane GO:0009925 ISO
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    lateral plasma membrane GO:0016328 ISO
    cytoplasmic vesicle membrane GO:0030659 ISO
    cell projection GO:0042995 IDA
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 IDA
    neuronal cell body GO:0043025 ISO
    cell body GO:0044297 IDA
    cell periphery GO:0071944 IDA
    cell periphery GO:0071944 ISO
 Experiment description of studies that identified Slc12a2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
4
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc12a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mdm2  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Sstr3  
Proximity Label-MS Mus musculus
4 Ezr 22350
Co-fractionation Mus musculus
5 Sppl2a  
Co-fractionation Mus musculus
6 Prnp 19122
Co-fractionation Mus musculus
7 Lamp2 16784
Co-fractionation Mus musculus
8 Stk39  
Affinity Capture-Western Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here