Gene description for Srsf2
Gene name serine/arginine-rich splicing factor 2
Gene symbol Srsf2
Other names/aliases D11Wsu175e
MRF-1
Pr264
SC35
Sfrs10
Sfrs2
Species Mus musculus
 Database cross references - Srsf2
ExoCarta ExoCarta_20382
Vesiclepedia VP_20382
Entrez Gene 20382
UniProt Q62093  
 Srsf2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Srsf2
Molecular Function
    RNA binding GO:0003723 IBA
    protein kinase C binding GO:0005080 IEA
    protein kinase C binding GO:0005080 ISO
    protein binding GO:0005515 IPI
    pre-mRNA binding GO:0036002 IDA
Biological Process
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IBA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IDA
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    response to vitamin E GO:0033197 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 IDA
    perichromatin fibrils GO:0005726 IEA
    perichromatin fibrils GO:0005726 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
    nuclear speck GO:0016607 ISO
    interchromatin granule GO:0035061 IEA
    interchromatin granule GO:0035061 ISO
 Experiment description of studies that identified Srsf2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Srsf2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbxo9  
Affinity Capture-RNA Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
3 Srrm1 51796
Co-fractionation Mus musculus
4 Fus  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
5 Gkap1  
Affinity Capture-RNA Mus musculus
6 Atxn1  
Affinity Capture-MS Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Zfp809  
Affinity Capture-RNA Mus musculus
9 Snrnp200 320632
Co-fractionation Mus musculus
10 Cwc22  
Co-fractionation Mus musculus
11 Ncl 17975
Co-fractionation Mus musculus
12 Sfi1  
Co-fractionation Mus musculus
13 Prpf3 70767
Co-fractionation Mus musculus
14 Nphp4  
Affinity Capture-MS Mus musculus
15 Zfp212  
Affinity Capture-RNA Mus musculus
16 Srsf1 110809
Co-fractionation Mus musculus
17 Srpk2 20817
Co-fractionation Mus musculus
18 Spen  
Co-fractionation Mus musculus
19 Htatsf1  
Co-fractionation Mus musculus
20 Npm1 18148
Co-localization Mus musculus
21 Slu7  
Co-fractionation Mus musculus
22 Kif13a  
Affinity Capture-RNA Mus musculus
23 Srsf3 20383
Co-fractionation Mus musculus
24 Mapt  
Affinity Capture-MS Mus musculus
25 Cir1  
Co-fractionation Mus musculus
26 Tia1  
Affinity Capture-MS Mus musculus
27 Ncbp2  
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here