Gene description for Prkg1
Gene name protein kinase, cGMP-dependent, type I
Gene symbol Prkg1
Other names/aliases AW125416
CGKI
Gm19690
Prkg1b
Prkgr1b
Species Mus musculus
 Database cross references - Prkg1
ExoCarta ExoCarta_19091
Entrez Gene 19091
UniProt P0C605  
 Prkg1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Prkg1
Molecular Function
    protein homodimerization activity GO:0042803 ISO
    cGMP-dependent protein kinase activity GO:0004692 ISO
    protein serine/threonine kinase activity GO:0004674 IDA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    mitogen-activated protein kinase p38 binding GO:0048273 ISO
    calcium channel regulator activity GO:0005246 ISO
    transferase activity GO:0016740 IEA
    nucleotide binding GO:0000166 IEA
    cGMP binding GO:0030553 IDA
    catalytic activity GO:0003824 IEA
    kinase activity GO:0016301 IEA
    protein kinase activity GO:0004672 IDA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
Biological Process
    phosphorylation GO:0016310 IEA
    positive regulation of circadian rhythm GO:0042753 ISO
    positive regulation of large conductance calcium-activated potassium channel activity GO:1902608 ISO
    positive regulation of vasodilation GO:0045909 ISO
    neuron migration GO:0001764 IMP
    cell growth involved in cardiac muscle cell development GO:0061049 ISO
    forebrain development GO:0030900 IMP
    cGMP-mediated signaling GO:0019934 IMP
    metabolic process GO:0008152 IEA
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 ISO
    regulation of testosterone biosynthetic process GO:2000224 ISO
    negative regulation of heart contraction GO:0045822 ISO
    relaxation of vascular smooth muscle GO:0060087 IMP
    negative regulation of platelet aggregation GO:0090331 ISO
    negative regulation of smooth muscle cell migration GO:0014912 ISO
    negative regulation of smooth muscle contraction GO:0045986 IMP
    signal transduction GO:0007165 IDA
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    protein phosphorylation GO:0006468 ISO
    dendrite development GO:0016358 IMP
    negative regulation of glutamate secretion GO:0014050 ISO
    regulation of GTPase activity GO:0043087 ISO
    negative regulation of inositol phosphate biosynthetic process GO:0010920 ISO
Subcellular Localization
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    acrosomal vesicle GO:0001669 ISO
    Golgi apparatus GO:0005794 ISO
    nucleoplasm GO:0005654 ISO
    intracellular GO:0005622 IMP
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Prkg1 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Prkg1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prkg1 is involved
PathwayEvidenceSource
Ca2+ pathway IEA Reactome
cGMP effects IEA Reactome
Rap1 signalling IEA Reactome





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