Gene description for AGER
Gene name advanced glycosylation end product-specific receptor
Gene symbol AGER
Other names/aliases RAGE
Species Homo sapiens
 Database cross references - AGER
ExoCarta ExoCarta_177
Vesiclepedia VP_177
Entrez Gene 177
HGNC 320
MIM 600214
UniProt Q15109  
 AGER identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for AGER
Molecular Function
    amyloid-beta binding GO:0001540 IDA
    amyloid-beta binding GO:0001540 IPI
    amyloid-beta binding GO:0001540 NAS
    amyloid-beta binding GO:0001540 TAS
    transmembrane signaling receptor activity GO:0004888 TAS
    scavenger receptor activity GO:0005044 TAS
    laminin receptor activity GO:0005055 IBA
    protein binding GO:0005515 IPI
    signaling receptor activity GO:0038023 IBA
    signaling receptor activity GO:0038023 IMP
    identical protein binding GO:0042802 IPI
    S100 protein binding GO:0044548 IPI
    protein-containing complex binding GO:0044877 IPI
    advanced glycation end-product receptor activity GO:0050785 NAS
    molecular adaptor activity GO:0060090 EXP
Biological Process
    response to hypoxia GO:0001666 IEA
    microglial cell activation GO:0001774 IGI
    microglial cell activation GO:0001774 ISS
    regulation of T cell mediated cytotoxicity GO:0001914 IDA
    positive regulation of protein phosphorylation GO:0001934 IGI
    inflammatory response GO:0006954 TAS
    cell adhesion GO:0007155 IEA
    cell surface receptor signaling pathway GO:0007166 TAS
    learning or memory GO:0007611 IGI
    response to wounding GO:0009611 TAS
    glucose mediated signaling pathway GO:0010255 IDA
    neuron projection development GO:0031175 IGI
    negative regulation of interleukin-10 production GO:0032693 IDA
    positive regulation of chemokine production GO:0032722 ISS
    positive regulation of interleukin-1 beta production GO:0032731 ISS
    positive regulation of interleukin-12 production GO:0032735 IMP
    positive regulation of interleukin-6 production GO:0032755 ISS
    positive regulation of tumor necrosis factor production GO:0032760 ISS
    positive regulation of heterotypic cell-cell adhesion GO:0034116 IGI
    positive regulation of activated T cell proliferation GO:0042104 IMP
    transcytosis GO:0045056 ISS
    positive regulation of JNK cascade GO:0046330 IGI
    astrocyte activation GO:0048143 IGI
    regulation of synaptic plasticity GO:0048167 TAS
    regulation of inflammatory response GO:0050727 IBA
    induction of positive chemotaxis GO:0050930 IEA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IGI
    positive regulation of monocyte chemotactic protein-1 production GO:0071639 IEA
    protein localization to membrane GO:0072657 IEA
    regulation of spontaneous synaptic transmission GO:0150003 IGI
    transport across blood-brain barrier GO:0150104 IDA
    transport across blood-brain barrier GO:0150104 NAS
    regulation of long-term synaptic potentiation GO:1900271 ISS
    negative regulation of long-term synaptic potentiation GO:1900272 IGI
    negative regulation of long-term synaptic potentiation GO:1900272 ISS
    negative regulation of long-term synaptic depression GO:1900453 IGI
    regulation of p38MAPK cascade GO:1900744 IBA
    regulation of p38MAPK cascade GO:1900744 ISS
    positive regulation of p38MAPK cascade GO:1900745 IGI
    regulation of non-canonical NF-kappaB signal transduction GO:1901222 NAS
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IGI
    positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process GO:1902961 IGI
    negative regulation of blood circulation GO:1903523 ISS
    positive regulation of endothelin production GO:1904472 ISS
    negative regulation of connective tissue replacement involved in inflammatory response wound healing GO:1904597 IEA
    response to amyloid-beta GO:1904645 ISS
    response to amyloid-beta GO:1904645 TAS
    cellular response to amyloid-beta GO:1904646 ISS
    positive regulation of monocyte extravasation GO:2000439 IMP
    regulation of CD4-positive, alpha-beta T cell activation GO:2000514 IDA
    positive regulation of dendritic cell differentiation GO:2001200 IMP
Subcellular Localization
    fibrillar center GO:0001650 IDA
    extracellular region GO:0005576 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 NAS
    apical plasma membrane GO:0016324 IDA
    cell junction GO:0030054 IDA
    postsynapse GO:0098794 TAS
 Experiment description of studies that identified AGER in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AGER
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 CREB3  
Two-hybrid Homo sapiens
3 NRP1 8829
Affinity Capture-MS Homo sapiens
4 CMTM7  
Two-hybrid Homo sapiens
5 S100P 6286
Affinity Capture-Western Homo sapiens
6 C3orf52  
Two-hybrid Homo sapiens
7 GRB2 2885
Affinity Capture-MS Homo sapiens
8 FPR2  
Affinity Capture-Western Homo sapiens
9 SEC61B 10952
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 FPR1  
Affinity Capture-Western Homo sapiens
11 CSTF2 1478
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AGER is involved
PathwayEvidenceSource
Advanced glycosylation endproduct receptor signaling TAS Reactome
Advanced glycosylation endproduct receptor signaling IEA Reactome
Cytokine Signaling in Immune system TAS Reactome
DDX58/IFIH1-mediated induction of interferon-alpha/beta TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Interleukin-1 family signaling TAS Reactome
Interleukin-1 signaling TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Signaling by Interleukins TAS Reactome
TAK1-dependent IKK and NF-kappa-B activation TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRAF6 mediated NF-kB activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome





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