Gene description for Mef2d
Gene name myocyte enhancer factor 2D
Gene symbol Mef2d
Other names/aliases C80750
Species Mus musculus
 Database cross references - Mef2d
ExoCarta ExoCarta_17261
Vesiclepedia VP_17261
Entrez Gene 17261
UniProt Q63943  
 Mef2d identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Mef2d
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 ISO
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISO
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA binding GO:0003677 IDA
    DNA-binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    protein homodimerization activity GO:0042803 IPI
    histone deacetylase binding GO:0042826 IDA
    histone deacetylase binding GO:0042826 ISO
    protein heterodimerization activity GO:0046982 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
    DNA-binding transcription factor binding GO:0140297 ISO
    sequence-specific double-stranded DNA binding GO:1990837 ISO
Biological Process
    osteoblast differentiation GO:0001649 IGI
    endochondral ossification GO:0001958 IGI
    chondrocyte differentiation GO:0002062 IGI
    regulation of DNA-templated transcription GO:0006355 IDA
    apoptotic process GO:0006915 IEA
    nervous system development GO:0007399 IEA
    adult heart development GO:0007512 IEA
    cell differentiation GO:0030154 IBA
    skeletal muscle cell differentiation GO:0035914 IMP
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IEA
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 ISO
Subcellular Localization
    nucleus GO:0005634 IC
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Mef2d in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Mef2d
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Runx2  
Affinity Capture-MS Mus musculus
2 KDM1A 23028
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 Kdm1a  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Mef2d is involved
PathwayEvidenceSource
Developmental Biology IEA Reactome
Myogenesis IEA Reactome





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