Experiment description of studies that identified Lgals3 in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 10 MISEV standards ✔ EM Biophysical techniques ✔ Alix|CD9|MHCII|MHCI|CD86|LAMP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] PubMed ID 11390481     Organism Mus musculus Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles. Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S." Journal name JIMMU Publication year 2001 Sample Dendritic cells Sample name D1 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.15-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] Western blotting

3 Experiment ID 14 MISEV standards ✔ EM Biophysical techniques ✔ RAB7|RAB11|CD9|CD63|LAMP1|LAMP2 Enriched markers ✔ DNM Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 16081791     Organism Mus musculus Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L" Journal name JIMMU Publication year 2005 Sample Microglia Sample name N9 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA XP] Western blotting

4 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Lgals3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Ncoa3   Two-hybrid Mus musculus 2 Lgals3 16854 Two-hybrid Mus musculus Two-hybrid Mus musculus 3 Traf6   Affinity Capture-MS Mus musculus Affinity Capture-Western Mus musculus 4 Sufu   Two-hybrid Mus musculus Reconstituted Complex Mus musculus Two-hybrid Mus musculus 5 Pias1   Two-hybrid Mus musculus 6 Cyhr1   Affinity Capture-Western Mus musculus Affinity Capture-Western Mus musculus 7 Atg5   Affinity Capture-MS Mus musculus 8 Cubn   Affinity Capture-Western Mus musculus View the network

Pathways in which Lgals3 is involved

No pathways found