Gene description for Klf4
Gene name Kruppel-like factor 4 (gut)
Gene symbol Klf4
Other names/aliases EZF
Gklf
Zie
Species Mus musculus
 Database cross references - Klf4
ExoCarta ExoCarta_16600
Vesiclepedia VP_16600
Entrez Gene 16600
UniProt Q60793  
 Klf4 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Klf4
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    transcription cis-regulatory region binding GO:0000976 ISO
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IDA
    RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity GO:0001010 IDA
    transcription coregulator binding GO:0001221 IEA
    transcription coregulator binding GO:0001221 ISO
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IMP
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISO
    DNA binding GO:0003677 ISS
    double-stranded DNA binding GO:0003690 ISO
    DNA-binding transcription factor activity GO:0003700 IDA
    DNA-binding transcription factor activity GO:0003700 ISO
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IPI
    chromatin DNA binding GO:0031490 IDA
    phosphatidylinositol 3-kinase regulator activity GO:0035014 ISO
    histone deacetylase binding GO:0042826 IEA
    histone deacetylase binding GO:0042826 ISO
    sequence-specific DNA binding GO:0043565 IDA
    sequence-specific DNA binding GO:0043565 ISO
    metal ion binding GO:0046872 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
    lncRNA binding GO:0106222 IDA
    sequence-specific double-stranded DNA binding GO:1990837 ISO
    promoter-specific chromatin binding GO:1990841 ISO
    promoter-specific chromatin binding GO:1990841 ISS
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    glandular epithelial cell differentiation GO:0002067 IMP
    defense response to tumor cell GO:0002357 IEA
    defense response to tumor cell GO:0002357 ISO
    DNA-templated transcription GO:0006351 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    transcription by RNA polymerase II GO:0006366 IMP
    negative regulation of cell population proliferation GO:0008285 IDA
    epidermis development GO:0008544 IMP
    epidermal cell differentiation GO:0009913 IMP
    gene expression GO:0010467 IMP
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of gene expression GO:0010629 ISO
    negative regulation of muscle hyperplasia GO:0014740 ISO
    negative regulation of angiogenesis GO:0016525 IEA
    negative regulation of angiogenesis GO:0016525 ISO
    stem cell population maintenance GO:0019827 IDA
    negative regulation of cell migration GO:0030336 IMP
    post-embryonic camera-type eye development GO:0031077 IMP
    negative regulation of NF-kappaB transcription factor activity GO:0032088 ISO
    positive regulation of telomere maintenance GO:0032206 IEA
    response to retinoic acid GO:0032526 IDA
    negative regulation of interleukin-8 production GO:0032717 IEA
    negative regulation of interleukin-8 production GO:0032717 ISO
    negative regulation of heterotypic cell-cell adhesion GO:0034115 IEA
    negative regulation of heterotypic cell-cell adhesion GO:0034115 ISO
    somatic stem cell population maintenance GO:0035019 IDA
    post-embryonic hemopoiesis GO:0035166 IEA
    post-embryonic hemopoiesis GO:0035166 ISO
    regulation of cell population proliferation GO:0042127 IGI
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IEA
    negative regulation of DNA-binding transcription factor activity GO:0043433 IMP
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISO
    fat cell differentiation GO:0045444 IMP
    fat cell differentiation GO:0045444 IMP
    regulation of cell differentiation GO:0045595 IDA
    negative regulation of DNA-templated transcription GO:0045892 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISS
    negative regulation of DNA-templated transcription GO:0045892 NAS
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    positive regulation of hemoglobin biosynthetic process GO:0046985 IEA
    positive regulation of hemoglobin biosynthetic process GO:0046985 ISO
    negative regulation of smooth muscle cell proliferation GO:0048662 IEA
    negative regulation of smooth muscle cell proliferation GO:0048662 ISO
    regulation of axon regeneration GO:0048679 IGI
    regulation of axon regeneration GO:0048679 IMP
    epidermis morphogenesis GO:0048730 IMP
    positive regulation of protein metabolic process GO:0051247 ISO
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 ISO
    canonical Wnt signaling pathway GO:0060070 IMP
    negative regulation of response to cytokine stimulus GO:0060761 IEA
    negative regulation of response to cytokine stimulus GO:0060761 ISO
    establishment of skin barrier GO:0061436 IMP
    cellular response to hydrogen peroxide GO:0070301 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    cellular response to retinoic acid GO:0071300 IEA
    cellular response to growth factor stimulus GO:0071363 IEA
    cellular response to growth factor stimulus GO:0071363 ISO
    cellular response to cycloheximide GO:0071409 IEA
    cellular response to laminar fluid shear stress GO:0071499 IEA
    cellular response to laminar fluid shear stress GO:0071499 ISO
    negative regulation of cell migration involved in sprouting angiogenesis GO:0090051 IEA
    negative regulation of cell migration involved in sprouting angiogenesis GO:0090051 ISO
    regulation of blastocyst development GO:0120222 IMP
    cellular response to peptide GO:1901653 IEA
    positive regulation of miRNA transcription GO:1902895 IEA
    positive regulation of miRNA transcription GO:1902895 ISO
    positive regulation of sprouting angiogenesis GO:1903672 IEA
    positive regulation of sprouting angiogenesis GO:1903672 ISO
    negative regulation of leukocyte adhesion to arterial endothelial cell GO:1904998 IEA
    negative regulation of leukocyte adhesion to arterial endothelial cell GO:1904998 ISO
    cellular response to leukemia inhibitory factor GO:1990830 IEP
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISO
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 IEA
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 ISO
    negative regulation of chemokine (C-X-C motif) ligand 2 production GO:2000342 IEA
    negative regulation of chemokine (C-X-C motif) ligand 2 production GO:2000342 ISO
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IEA
Subcellular Localization
    chromatin GO:0000785 ISO
    euchromatin GO:0000791 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IPI
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IDA
    transcription regulator complex GO:0005667 ISO
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    microtubule cytoskeleton GO:0015630 IEA
    microtubule cytoskeleton GO:0015630 ISO
 Experiment description of studies that identified Klf4 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Klf4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Trp53  
Affinity Capture-Western Mus musculus
2 Zc3h12a  
Affinity Capture-Western Mus musculus
3 Rad21  
Co-localization Mus musculus
4 Usp7  
Affinity Capture-MS Mus musculus
5 Tsix  
Affinity Capture-RNA Mus musculus
6 Smarca4  
Affinity Capture-Western Mus musculus
7 Pou5f1  
Affinity Capture-MS Mus musculus
8 TP53 7157
Affinity Capture-Western Homo sapiens
9 Apc  
Phenotypic Enhancement Mus musculus
View the network image/svg+xml
 Pathways in which Klf4 is involved
No pathways found





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