Gene description for Itgb3
Gene name integrin beta 3
Gene symbol Itgb3
Other names/aliases CD61
GP3A
INGRB3
Species Mus musculus
 Database cross references - Itgb3
ExoCarta ExoCarta_16416
Vesiclepedia VP_16416
Entrez Gene 16416
UniProt O54890  
 Itgb3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Itgb3
Molecular Function
    fibronectin binding GO:0001968 IBA
    fibronectin binding GO:0001968 IEA
    fibronectin binding GO:0001968 ISO
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    protein disulfide isomerase activity GO:0003756 IEA
    protein disulfide isomerase activity GO:0003756 ISO
    protein kinase C binding GO:0005080 IPI
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 IPI
    integrin binding GO:0005178 ISO
    protein binding GO:0005515 IPI
    fibroblast growth factor binding GO:0017134 IEA
    fibroblast growth factor binding GO:0017134 ISO
    enzyme binding GO:0019899 IPI
    enzyme binding GO:0019899 ISO
    C-X3-C chemokine binding GO:0019960 ISO
    C-X3-C chemokine binding GO:0019960 ISS
    insulin-like growth factor I binding GO:0031994 IEA
    insulin-like growth factor I binding GO:0031994 ISO
    neuregulin binding GO:0038132 ISO
    neuregulin binding GO:0038132 ISS
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    vascular endothelial growth factor receptor 2 binding GO:0043184 IEA
    vascular endothelial growth factor receptor 2 binding GO:0043184 ISO
    metal ion binding GO:0046872 IEA
    cell adhesion molecule binding GO:0050839 ISO
    extracellular matrix binding GO:0050840 IEA
    extracellular matrix binding GO:0050840 ISO
    fibrinogen binding GO:0070051 IBA
    fibrinogen binding GO:0070051 IEA
    fibrinogen binding GO:0070051 ISO
Biological Process
    positive regulation of endothelial cell proliferation GO:0001938 IEA
    positive regulation of endothelial cell proliferation GO:0001938 ISO
    positive regulation of cell-matrix adhesion GO:0001954 IMP
    positive regulation of leukocyte migration GO:0002687 ISO
    cell-substrate junction assembly GO:0007044 IDA
    cell-matrix adhesion GO:0007160 IBA
    cell-matrix adhesion GO:0007160 IGI
    cell-matrix adhesion GO:0007160 ISO
    cell-matrix adhesion GO:0007160 NAS
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 NAS
    embryo implantation GO:0007566 IEA
    regulation of G protein-coupled receptor signaling pathway GO:0008277 ISO
    positive regulation of endothelial cell migration GO:0010595 IEA
    positive regulation of endothelial cell migration GO:0010595 ISO
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of macrophage derived foam cell differentiation GO:0010745 IEA
    negative regulation of macrophage derived foam cell differentiation GO:0010745 ISO
    positive regulation of fibroblast migration GO:0010763 IMP
    positive regulation of fibroblast migration GO:0010763 ISO
    negative regulation of lipid storage GO:0010888 IEA
    negative regulation of lipid storage GO:0010888 ISO
    response to activity GO:0014823 IEA
    smooth muscle cell migration GO:0014909 IEA
    smooth muscle cell migration GO:0014909 ISO
    positive regulation of smooth muscle cell migration GO:0014911 IEA
    positive regulation of smooth muscle cell migration GO:0014911 ISO
    cell migration GO:0016477 IBA
    platelet activation GO:0030168 ISO
    positive regulation of cell migration GO:0030335 IMP
    cell-substrate adhesion GO:0031589 ISO
    activation of protein kinase activity GO:0032147 ISO
    negative regulation of lipid transport GO:0032369 IEA
    negative regulation of lipid transport GO:0032369 ISO
    regulation of protein localization GO:0032880 IMP
    regulation of actin cytoskeleton organization GO:0032956 IMP
    cell adhesion mediated by integrin GO:0033627 IBA
    cell adhesion mediated by integrin GO:0033627 ISO
    cell adhesion mediated by integrin GO:0033627 ISS
    positive regulation of cell adhesion mediated by integrin GO:0033630 IEA
    positive regulation of cell adhesion mediated by integrin GO:0033630 ISO
    positive regulation of osteoblast proliferation GO:0033690 IMP
    heterotypic cell-cell adhesion GO:0034113 IEA
    heterotypic cell-cell adhesion GO:0034113 ISO
    substrate adhesion-dependent cell spreading GO:0034446 IEA
    substrate adhesion-dependent cell spreading GO:0034446 ISO
    cellular response to platelet-derived growth factor stimulus GO:0036120 IMP
    apolipoprotein A-I-mediated signaling pathway GO:0038027 IEA
    apolipoprotein A-I-mediated signaling pathway GO:0038027 ISO
    apoptotic cell clearance GO:0043277 IGI
    apoptotic cell clearance GO:0043277 ISO
    positive regulation of osteoclast differentiation GO:0045672 IC
    positive regulation of angiogenesis GO:0045766 IEA
    positive regulation of angiogenesis GO:0045766 ISO
    positive regulation of bone resorption GO:0045780 IC
    positive regulation of bone resorption GO:0045780 ISO
    symbiont entry into host cell GO:0046718 IEA
    symbiont entry into host cell GO:0046718 ISO
    platelet-derived growth factor receptor signaling pathway GO:0048008 IMP
    positive regulation of fibroblast proliferation GO:0048146 IMP
    mesodermal cell differentiation GO:0048333 IEA
    positive regulation of smooth muscle cell proliferation GO:0048661 IEA
    positive regulation of smooth muscle cell proliferation GO:0048661 ISO
    cell projection morphogenesis GO:0048858 IC
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    negative regulation of lipoprotein metabolic process GO:0050748 IEA
    negative regulation of lipoprotein metabolic process GO:0050748 ISO
    negative chemotaxis GO:0050919 IEA
    negative chemotaxis GO:0050919 ISO
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IEA
    regulation of release of sequestered calcium ion into cytosol GO:0051279 ISO
    regulation of serotonin uptake GO:0051611 IMP
    regulation of protein tyrosine kinase activity GO:0061097 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    platelet aggregation GO:0070527 IBA
    platelet aggregation GO:0070527 IMP
    platelet aggregation GO:0070527 ISO
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular response to xenobiotic stimulus GO:0071466 IEA
    positive regulation of glomerular mesangial cell proliferation GO:0072126 IEA
    positive regulation of glomerular mesangial cell proliferation GO:0072126 ISO
    blood coagulation, fibrin clot formation GO:0072378 IMP
    maintenance of postsynaptic specialization structure GO:0098880 IEA
    maintenance of postsynaptic specialization structure GO:0098880 ISO
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IEA
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 ISO
    regulation of postsynaptic neurotransmitter receptor diffusion trapping GO:0150054 IEA
    regulation of postsynaptic neurotransmitter receptor diffusion trapping GO:0150054 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IMP
    positive regulation of adenylate cyclase-inhibiting opioid receptor signaling pathway GO:1900731 IEA
    positive regulation of adenylate cyclase-inhibiting opioid receptor signaling pathway GO:1900731 ISO
    regulation of trophoblast cell migration GO:1901163 IMP
    regulation of extracellular matrix organization GO:1903053 IMP
    negative regulation of low-density lipoprotein receptor activity GO:1905598 ISO
    cellular response to insulin-like growth factor stimulus GO:1990314 IEA
    negative regulation of endothelial cell apoptotic process GO:2000352 IEA
    negative regulation of endothelial cell apoptotic process GO:2000352 ISO
    positive regulation of T cell migration GO:2000406 IDA
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IEA
    cell-cell junction GO:0005911 ISO
    focal adhesion GO:0005925 IDA
    focal adhesion GO:0005925 ISO
    integrin complex GO:0008305 IBA
    integrin complex GO:0008305 ISO
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    lamellipodium membrane GO:0031258 IEA
    lamellipodium membrane GO:0031258 ISO
    filopodium membrane GO:0031527 IEA
    filopodium membrane GO:0031527 ISO
    microvillus membrane GO:0031528 IEA
    microvillus membrane GO:0031528 ISO
    ruffle membrane GO:0032587 IEA
    ruffle membrane GO:0032587 ISO
    protein-containing complex GO:0032991 ISO
    integrin alpha9-beta1 complex GO:0034679 TAS
    integrin alphav-beta3 complex GO:0034683 IEA
    integrin alphav-beta3 complex GO:0034683 ISO
    alphav-beta3 integrin-PKCalpha complex GO:0035866 IDA
    alphav-beta3 integrin-PKCalpha complex GO:0035866 ISO
    alphav-beta3 integrin-IGF-1-IGF1R complex GO:0035867 IEA
    alphav-beta3 integrin-IGF-1-IGF1R complex GO:0035867 ISO
    alphav-beta3 integrin-HMGB1 complex GO:0035868 IEA
    alphav-beta3 integrin-HMGB1 complex GO:0035868 ISO
    melanosome GO:0042470 IEA
    melanosome GO:0042470 ISO
    receptor complex GO:0043235 ISO
    synapse GO:0045202 IBA
    synapse GO:0045202 IDA
    postsynaptic membrane GO:0045211 IEA
    integrin alphaIIb-beta3 complex GO:0070442 ISO
    alpha9-beta1 integrin-ADAM8 complex GO:0071133 IDA
    synaptic membrane GO:0097060 IDA
    synaptic membrane GO:0097060 IEP
    glycinergic synapse GO:0098690 IEA
    glycinergic synapse GO:0098690 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Itgb3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Itgb3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pla2g4a  
Affinity Capture-Western Mus musculus
2 Csk 12988
Affinity Capture-Western Mus musculus
3 Prkca 18750
Affinity Capture-Western Mus musculus
4 Ubqln2  
Reconstituted Complex Mus musculus
5 Prkcb  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Itgb3 is involved
PathwayEvidenceSource
Axon guidance IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Developmental Biology IEA Reactome
ECM proteoglycans IEA Reactome
Elastic fibre formation IEA Reactome
Extracellular matrix organization IEA Reactome
GRB2:SOS provides linkage to MAPK signaling for Integrins IEA Reactome
Hemostasis IEA Reactome
Integrin cell surface interactions IEA Reactome
Integrin signaling IEA Reactome
L1CAM interactions IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
Molecules associated with elastic fibres IEA Reactome
Nervous system development IEA Reactome
Non-integrin membrane-ECM interactions IEA Reactome
p130Cas linkage to MAPK signaling for integrins IEA Reactome
PECAM1 interactions IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet Aggregation (Plug Formation) IEA Reactome
Platelet degranulation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Response to elevated platelet cytosolic Ca2+ IEA Reactome
Signal Transduction IEA Reactome
Signal transduction by L1 IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by TGF-beta Receptor Complex IEA Reactome
Signaling by TGFB family members IEA Reactome
Signaling by VEGF IEA Reactome
Syndecan interactions IEA Reactome
TGF-beta receptor signaling activates SMADs IEA Reactome
Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome





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