Gene description for Inpp5d
Gene name inositol polyphosphate-5-phosphatase D
Gene symbol Inpp5d
Other names/aliases SHIP
SHIP-1
SHIP1
SIP-145
p150Ship
s-SHIP
Species Mus musculus
 Database cross references - Inpp5d
ExoCarta ExoCarta_16331
Vesiclepedia VP_16331
Entrez Gene 16331
UniProt Q9ES52  
 Inpp5d identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Inpp5d
Molecular Function
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    inositol-4,5-bisphosphate 5-phosphatase activity GO:0030487 ISO
    phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity GO:0034485 IEA
    inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity GO:0052659 IEA
Biological Process
    apoptotic process GO:0006915 IEA
    determination of adult lifespan GO:0008340 IMP
    negative regulation of signal transduction GO:0009968 IBA
    negative regulation of signal transduction GO:0009968 IMP
    immunoglobulin mediated immune response GO:0016064 IMP
    negative regulation of granulocyte differentiation GO:0030853 IMP
    negative regulation of B cell proliferation GO:0030889 IMP
    negative regulation of interleukin-6 production GO:0032715 IMP
    intracellular signal transduction GO:0035556 IMP
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of B cell differentiation GO:0045579 IBA
    positive regulation of B cell differentiation GO:0045579 IMP
    positive regulation of lymphocyte differentiation GO:0045621 IMP
    positive regulation of erythrocyte differentiation GO:0045648 IMP
    negative regulation of monocyte differentiation GO:0045656 IMP
    negative regulation of neutrophil differentiation GO:0045659 IBA
    negative regulation of neutrophil differentiation GO:0045659 IMP
    negative regulation of osteoclast differentiation GO:0045671 IMP
    negative regulation of bone resorption GO:0045779 IBA
    negative regulation of bone resorption GO:0045779 IMP
    phosphatidylinositol dephosphorylation GO:0046856 IEA
    regulation of immune response GO:0050776 IBA
    negative regulation of immune response GO:0050777 IMP
    negative regulation of B cell activation GO:0050869 IMP
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    actin filament GO:0005884 ISO
    plasma membrane GO:0005886 IEA
    cortical cytoskeleton GO:0030863 ISO
    membrane raft GO:0045121 IEA
 Experiment description of studies that identified Inpp5d in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Inpp5d
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pecam1 18613
Co-fractionation Mus musculus
2 Rsu1 20163
Affinity Capture-MS Mus musculus
3 Ctnnd1 12388
Affinity Capture-MS Mus musculus
4 Pstpip1 19200
Affinity Capture-MS Mus musculus
5 Foxp3  
Affinity Capture-MS Mus musculus
6 Shc1  
Affinity Capture-MS Mus musculus
Far Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
7 Dok1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
8 Btk  
Affinity Capture-MS Mus musculus
9 Dok2  
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
10 Tmem173  
Proximity Label-MS Mus musculus
11 Dctn2 69654
Affinity Capture-MS Mus musculus
12 Hck  
Co-fractionation Mus musculus
13 Dok3  
Affinity Capture-MS Mus musculus
14 Src 20779
Affinity Capture-Western Mus musculus
15 Parvg  
Affinity Capture-MS Mus musculus
16 Sh3kbp1 58194
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
17 Abl1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
18 Actr1a 54130
Affinity Capture-MS Mus musculus
19 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
20 Sec23a  
Affinity Capture-MS Mus musculus
21 Crkl 12929
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
22 Bcar1  
Affinity Capture-MS Mus musculus
23 Atg16l1  
Affinity Capture-MS Mus musculus
24 Lpxn  
Affinity Capture-MS Mus musculus
25 Cbl 12402
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
26 Fancd2  
Affinity Capture-MS Mus musculus
27 Ptpn11 19247
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
28 Tjp2 21873
Affinity Capture-MS Mus musculus
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