Gene description for DBH
Gene name dopamine beta-hydroxylase (dopamine beta-monooxygenase)
Gene symbol DBH
Other names/aliases DBM
Species Homo sapiens
 Database cross references - DBH
ExoCarta ExoCarta_1621
Vesiclepedia VP_1621
Entrez Gene 1621
HGNC 2689
MIM 609312
UniProt P09172  
 DBH identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for DBH
Molecular Function
    catalytic activity GO:0003824 TAS
    dopamine beta-monooxygenase activity GO:0004500 IBA
    dopamine beta-monooxygenase activity GO:0004500 IDA
    copper ion binding GO:0005507 IBA
    copper ion binding GO:0005507 IDA
    protein binding GO:0005515 IPI
    L-ascorbic acid binding GO:0031418 IEA
Biological Process
    blood vessel remodeling GO:0001974 IEA
    response to amphetamine GO:0001975 IEA
    leukocyte mediated immunity GO:0002443 IEA
    octopamine biosynthetic process GO:0006589 IBA
    chemical synaptic transmission GO:0007268 TAS
    memory GO:0007613 IEA
    locomotory behavior GO:0007626 IEA
    visual learning GO:0008542 IEA
    homoiothermy GO:0042309 IEA
    vasoconstriction GO:0042310 IEA
    dopamine catabolic process GO:0042420 IBA
    dopamine catabolic process GO:0042420 IDA
    norepinephrine biosynthetic process GO:0042421 IBA
    norepinephrine biosynthetic process GO:0042421 IEA
    norepinephrine biosynthetic process GO:0042421 IMP
    glucose homeostasis GO:0042593 IEA
    fear response GO:0042596 IEA
    maternal behavior GO:0042711 IEA
    positive regulation of vasoconstriction GO:0045907 IEA
    behavioral response to ethanol GO:0048149 IEA
    response to pain GO:0048265 IEA
    leukocyte migration GO:0050900 IEA
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    regulation of vascular associated smooth muscle cell proliferation GO:1904705 IEA
    regulation of vascular endothelial cell proliferation GO:1905562 IEA
    regulation of extrinsic apoptotic signaling pathway GO:2001236 IEA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    cytoplasm GO:0005737 TAS
    endoplasmic reticulum GO:0005783 IDA
    membrane GO:0016020 TAS
    transport vesicle membrane GO:0030658 IEA
    secretory granule membrane GO:0030667 IBA
    secretory granule membrane GO:0030667 IDA
    centriolar satellite GO:0034451 IDA
    chromaffin granule lumen GO:0034466 IEA
    secretory granule lumen GO:0034774 IDA
    secretory granule lumen GO:0034774 TAS
    chromaffin granule membrane GO:0042584 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    synapse GO:0045202 IEA
 Experiment description of studies that identified DBH in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for DBH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NOTCH2NL 388677
Two-hybrid Homo sapiens
2 PPT1 5538
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 NBPF19  
Two-hybrid Homo sapiens
4 LEMD3  
Affinity Capture-MS Homo sapiens
5 NTRK1 4914
Affinity Capture-MS Homo sapiens
6 RPL23 9349
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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