Gene description for Ptpn6
Gene name protein tyrosine phosphatase, non-receptor type 6
Gene symbol Ptpn6
Other names/aliases 70Z-SHP
Hcph
PTPTY-42
Ptp1C
SH-PTP1
SHP-1
hcp
me
Species Mus musculus
 Database cross references - Ptpn6
ExoCarta ExoCarta_15170
Vesiclepedia VP_15170
Entrez Gene 15170
UniProt P29351  
 Ptpn6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ptpn6
Molecular Function
    phosphotyrosine residue binding GO:0001784 IBA
    phosphotyrosine residue binding GO:0001784 IPI
    phosphotyrosine residue binding GO:0001784 ISO
    protein tyrosine phosphatase activity GO:0004725 IDA
    protein tyrosine phosphatase activity GO:0004725 IMP
    protein tyrosine phosphatase activity GO:0004725 ISO
    protein tyrosine phosphatase activity GO:0004725 ISS
    protein tyrosine phosphatase activity GO:0004725 TAS
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IBA
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    transmembrane receptor protein tyrosine phosphatase activity GO:0005001 ISO
    transmembrane receptor protein tyrosine phosphatase activity GO:0005001 ISS
    cytokine receptor binding GO:0005126 ISO
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IDA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    SH2 domain binding GO:0042169 IDA
    natural killer cell lectin-like receptor binding GO:0046703 ISO
    cell adhesion molecule binding GO:0050839 IPI
    phosphorylation-dependent protein binding GO:0140031 IEA
    phosphorylation-dependent protein binding GO:0140031 ISO
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    MAPK cascade GO:0000165 IBA
    MAPK cascade GO:0000165 IMP
    mitotic cell cycle GO:0000278 IBA
    hematopoietic progenitor cell differentiation GO:0002244 IMP
    negative regulation of humoral immune response mediated by circulating immunoglobulin GO:0002924 IMP
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 IDA
    protein dephosphorylation GO:0006470 IMP
    protein dephosphorylation GO:0006470 ISO
    positive regulation of cell population proliferation GO:0008284 IEA
    positive regulation of cell population proliferation GO:0008284 ISO
    negative regulation of angiogenesis GO:0016525 IEA
    negative regulation of angiogenesis GO:0016525 ISO
    peptidyl-tyrosine phosphorylation GO:0018108 IMP
    peptidyl-tyrosine phosphorylation GO:0018108 ISO
    cytokine-mediated signaling pathway GO:0019221 TAS
    cell differentiation GO:0030154 IBA
    cell differentiation GO:0030154 ISO
    cell differentiation GO:0030154 ISS
    platelet formation GO:0030220 IGI
    negative regulation of lipopolysaccharide-mediated signaling pathway GO:0031665 IGI
    negative regulation of interleukin-6 production GO:0032715 IGI
    negative regulation of tumor necrosis factor production GO:0032720 IGI
    negative regulation of mast cell activation involved in immune response GO:0033007 IDA
    positive regulation of cell adhesion mediated by integrin GO:0033630 IMP
    peptidyl-tyrosine dephosphorylation GO:0035335 ISO
    intracellular signal transduction GO:0035556 IDA
    megakaryocyte development GO:0035855 IMP
    T cell proliferation GO:0042098 IMP
    negative regulation of T cell proliferation GO:0042130 IMP
    natural killer cell mediated cytotoxicity GO:0042267 IMP
    negative regulation of MAP kinase activity GO:0043407 IMP
    negative regulation of MAPK cascade GO:0043409 IMP
    regulation of B cell differentiation GO:0045577 IMP
    negative regulation of innate immune response GO:0045824 IEA
    negative regulation of peptidyl-tyrosine phosphorylation GO:0050732 IMP
    negative regulation of peptidyl-tyrosine phosphorylation GO:0050732 ISO
    T cell receptor signaling pathway GO:0050852 IMP
    B cell receptor signaling pathway GO:0050853 IGI
    B cell receptor signaling pathway GO:0050853 IMP
    negative regulation of B cell receptor signaling pathway GO:0050859 IEA
    negative regulation of T cell receptor signaling pathway GO:0050860 IMP
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IGI
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    regulation of ERK1 and ERK2 cascade GO:0070372 ISO
    regulation of ERK1 and ERK2 cascade GO:0070372 ISS
    platelet aggregation GO:0070527 IMP
    negative regulation of inflammatory response to wounding GO:0106015 IDA
    negative regulation of inflammatory response to wounding GO:0106015 ISO
    CD27 signaling pathway GO:0160162 IEA
    epididymis development GO:1905867 IMP
    regulation of G1/S transition of mitotic cell cycle GO:2000045 IEA
    regulation of G1/S transition of mitotic cell cycle GO:2000045 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IDA
    protein-containing complex GO:0032991 ISO
    alpha-beta T cell receptor complex GO:0042105 IDA
    apical dendrite GO:0097440 ISO
 Experiment description of studies that identified Ptpn6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ptpn6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cblb 208650
Affinity Capture-Western Mus musculus
2 Ptprm  
Co-fractionation Mus musculus
3 Ncoa3  
Two-hybrid Mus musculus
4 NR5A2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
5 Tmem173  
Proximity Label-MS Mus musculus
6 Ptprq  
Co-fractionation Mus musculus
7 Ptprf  
Co-fractionation Mus musculus
8 Ptpn3  
Co-fractionation Mus musculus
9 Ptprs 19280
Co-fractionation Mus musculus
10 Ptpn13  
Co-fractionation Mus musculus
11 Dok1  
Biochemical Activity Mus musculus
12 Cd247  
Affinity Capture-Western Mus musculus
13 Ptpra 19262
Co-fractionation Mus musculus
14 Fancd2  
Affinity Capture-MS Mus musculus
15 Traf3  
Affinity Capture-Western Mus musculus
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