Gene description for Eif4a2
Gene name eukaryotic translation initiation factor 4A2
Gene symbol Eif4a2
Other names/aliases 4833432N07Rik
BM-010
Ddx2b
Eif4
eIF-4A-II
eIF4A-II
Species Mus musculus
 Database cross references - Eif4a2
ExoCarta ExoCarta_13682
Entrez Gene 13682
UniProt P10630  
 Eif4a2 identified in exosomes derived from the following tissue/cell type
Dendritic cells 11390481    
Microglia 16081791    
 Gene ontology annotations for Eif4a2
Molecular Function
    hydrolase activity GO:0016787 IEA
    nucleotide binding GO:0000166 IEA
    RNA binding GO:0003723 IEA
    ATP-dependent RNA helicase activity GO:0004004 IBA
    helicase activity GO:0004386 IEA
    translation initiation factor activity GO:0003743 IEA
    poly(A) RNA binding GO:0044822 ISO
    ATP binding GO:0005524 IEA
    nucleic acid binding GO:0003676 IEA
    ATPase activity GO:0016887 ISO
Biological Process
    translational initiation GO:0006413 IBA
    regulation of gene expression GO:0010468 IBA
    RNA secondary structure unwinding GO:0010501 IBA
    negative regulation of RNA-directed RNA polymerase activity GO:1900260 ISO
    translation GO:0006412 IEA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Eif4a2 in exosomes
1
Experiment ID 10
ISEV standards
EM
EV Biophysical techniques
Alix
EV Cytosolic markers
CD9|MHCII|MHCI|CD86|LAMP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S.
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 14
ISEV standards
EM
EV Biophysical techniques
RAB7|RAB11
EV Cytosolic markers
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
DNM
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Eif4a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX24 57062
Two-hybrid Homo sapiens
View the network image/svg+xml



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