Gene description for Col2a1
Gene name collagen, type II, alpha 1
Gene symbol Col2a1
Other names/aliases Col2
Col2a
Col2a-1
Del1
Dmm
Lpk
M100856
Rgsc856
Species Mus musculus
 Database cross references - Col2a1
ExoCarta ExoCarta_12824
Vesiclepedia VP_12824
Entrez Gene 12824
UniProt P28481  
 Col2a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Col2a1
Molecular Function
    extracellular matrix structural constituent GO:0005201 IEA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IBA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
    MHC class II protein binding GO:0042289 IEA
    MHC class II protein binding GO:0042289 ISO
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    proteoglycan binding GO:0043394 ISO
    metal ion binding GO:0046872 IEA
    platelet-derived growth factor binding GO:0048407 ISO
Biological Process
    skeletal system development GO:0001501 ISO
    cartilage condensation GO:0001502 IMP
    ossification GO:0001503 IEP
    tissue homeostasis GO:0001894 IMP
    endochondral ossification GO:0001958 IMP
    chondrocyte differentiation GO:0002062 IEP
    chondrocyte differentiation GO:0002062 IMP
    heart morphogenesis GO:0003007 IMP
    proteoglycan metabolic process GO:0006029 IMP
    central nervous system development GO:0007417 IEP
    visual perception GO:0007601 IEA
    visual perception GO:0007601 ISO
    sensory perception of sound GO:0007605 IEA
    sensory perception of sound GO:0007605 ISO
    regulation of gene expression GO:0010468 IMP
    extracellular matrix organization GO:0030198 IBA
    collagen fibril organization GO:0030199 IMP
    collagen fibril organization GO:0030199 ISO
    notochord development GO:0030903 IEP
    limb morphogenesis GO:0035108 IMP
    inner ear morphogenesis GO:0042472 IMP
    skeletal system morphogenesis GO:0048705 IMP
    inner ear development GO:0048839 IMP
    cartilage development GO:0051216 IMP
    roof of mouth development GO:0060021 IMP
    limb bud formation GO:0060174 IEP
    embryonic skeletal joint morphogenesis GO:0060272 IEA
    embryonic skeletal joint morphogenesis GO:0060272 ISO
    bone development GO:0060348 IMP
    cartilage development involved in endochondral bone morphogenesis GO:0060351 IMP
    otic vesicle development GO:0071599 IEP
    cellular response to BMP stimulus GO:0071773 IDA
    anterior head development GO:0097065 IMP
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IMP
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IMP
Subcellular Localization
    collagen trimer GO:0005581 IDA
    collagen type II trimer GO:0005585 IBA
    collagen type II trimer GO:0005585 IDA
    collagen type II trimer GO:0005585 IMP
    collagen type II trimer GO:0005585 ISO
    collagen type XI trimer GO:0005592 NAS
    basement membrane GO:0005604 IDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IDA
    extracellular matrix GO:0031012 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 ISO
    collagen-containing extracellular matrix GO:0062023 NAS
 Experiment description of studies that identified Col2a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Col2a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Enam  
Co-fractionation Mus musculus
2 Col11a1  
Co-fractionation Mus musculus
3 Col16a1 107581
Co-fractionation Mus musculus
4 Col4a1 12826
Co-fractionation Mus musculus
5 Loxl4 67573
Co-fractionation Mus musculus
6 Comp  
Co-fractionation Mus musculus
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