Gene description for Aprt
Gene name adenine phosphoribosyl transferase
Gene symbol Aprt
Other names/aliases C85684
Species Mus musculus
 Database cross references - Aprt
ExoCarta ExoCarta_11821
Vesiclepedia VP_11821
Entrez Gene 11821
UniProt P08030  
 Aprt identified in sEVs derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Aprt
Molecular Function
    adenine binding GO:0002055 IBA
    adenine binding GO:0002055 IDA
    adenine phosphoribosyltransferase activity GO:0003999 IBA
    adenine phosphoribosyltransferase activity GO:0003999 IDA
    adenine phosphoribosyltransferase activity GO:0003999 IGI
    adenine phosphoribosyltransferase activity GO:0003999 IMP
    adenine phosphoribosyltransferase activity GO:0003999 ISO
    AMP binding GO:0016208 IBA
    AMP binding GO:0016208 ISO
Biological Process
    purine ribonucleoside salvage GO:0006166 IGI
    purine ribonucleoside salvage GO:0006166 IMP
    adenine salvage GO:0006168 IBA
    adenine salvage GO:0006168 IEA
    lactation GO:0007595 ISO
    grooming behavior GO:0007625 IGI
    grooming behavior GO:0007625 IGI
    GMP salvage GO:0032263 IDA
    IMP salvage GO:0032264 IMP
    AMP salvage GO:0044209 IBA
    AMP salvage GO:0044209 IDA
    AMP salvage GO:0044209 IEA
    AMP salvage GO:0044209 IMP
    AMP salvage GO:0044209 IMP
    adenine metabolic process GO:0046083 IC
    adenine metabolic process GO:0046083 IGI
    adenine metabolic process GO:0046083 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Aprt in sEVs
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Aprt
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRKAA1 5562
Two-hybrid Homo sapiens
2 Eed  
Affinity Capture-MS Mus musculus
3 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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