Gene description for Actc1
Gene name actin, alpha, cardiac muscle 1
Gene symbol Actc1
Other names/aliases Actc-1
Species Mus musculus
 Database cross references - Actc1
ExoCarta ExoCarta_11464
Vesiclepedia VP_11464
Entrez Gene 11464
UniProt P68033  
 Actc1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Actc1
Molecular Function
    microfilament motor activity GO:0000146 IEA
    microfilament motor activity GO:0000146 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    hydrolase activity GO:0016787 IEA
    myosin binding GO:0017022 IBA
    myosin binding GO:0017022 IEA
    myosin binding GO:0017022 ISO
Biological Process
    actin filament organization GO:0007015 IBA
    response to xenobiotic stimulus GO:0009410 IEA
    positive regulation of gene expression GO:0010628 ISS
    actin filament-based movement GO:0030048 ISO
    skeletal muscle thin filament assembly GO:0030240 IMP
    actomyosin structure organization GO:0031032 IMP
    actin-myosin filament sliding GO:0033275 IBA
    actin-myosin filament sliding GO:0033275 IEA
    actin-myosin filament sliding GO:0033275 ISO
    negative regulation of apoptotic process GO:0043066 IMP
    response to ethanol GO:0045471 IEA
    cardiac myofibril assembly GO:0055003 IMP
    cardiac muscle tissue morphogenesis GO:0055008 IMP
    heart contraction GO:0060047 IBA
    heart contraction GO:0060047 ISO
    cardiac muscle contraction GO:0060048 IEA
    cardiac muscle contraction GO:0060048 ISO
    actin-mediated cell contraction GO:0070252 IMP
    mesenchyme migration GO:0090131 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISO
    actin filament GO:0005884 IBA
    actin filament GO:0005884 IEA
    actin filament GO:0005884 ISO
    actin cytoskeleton GO:0015629 IBA
    sarcomere GO:0030017 IBA
    sarcomere GO:0030017 ISO
    lamellipodium GO:0030027 ISS
    filopodium GO:0030175 ISS
    I band GO:0031674 IDA
    cell body GO:0044297 ISS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
 Experiment description of studies that identified Actc1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Actc1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nphp4  
Affinity Capture-MS Mus musculus
2 Grin2b  
Affinity Capture-Western Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Tfe3  
Affinity Capture-MS Mus musculus
5 Mapt  
Affinity Capture-MS Mus musculus
6 Dmrt2  
Affinity Capture-MS Mus musculus
7 Grin1  
Affinity Capture-Western Mus musculus
8 HACE1 57531
Affinity Capture-MS Homo sapiens
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