Gene description for PLA2G16
Gene name phospholipase A2, group XVI
Gene symbol PLA2G16
Other names/aliases AdPLA
H-REV107-1
HRASLS3
HREV107
HREV107-1
HREV107-3
HRSL3
Species Homo sapiens
 Database cross references - PLA2G16
ExoCarta ExoCarta_11145
Vesiclepedia VP_11145
Entrez Gene 11145
HGNC 17825
MIM 613867
UniProt P53816  
 PLA2G16 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PLA2G16
Molecular Function
    phospholipase A2 activity GO:0004623 IBA
    phospholipase A2 activity GO:0004623 IDA
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 EXP
    phospholipase A1 activity GO:0008970 IBA
    phospholipase A1 activity GO:0008970 IDA
    N-acyltransferase activity GO:0016410 IBA
    N-acyltransferase activity GO:0016410 IDA
    acyltransferase activity GO:0016746 TAS
    calcium-dependent phospholipase A2 activity GO:0047498 IEA
    calcium-independent phospholipase A2 activity GO:0047499 IEA
    phosphatidylserine 1-acylhydrolase activity GO:0052739 IEA
Biological Process
    triglyceride metabolic process GO:0006641 ISS
    phospholipid metabolic process GO:0006644 IDA
    peroxisome organization GO:0007031 ISS
    phospholipid biosynthetic process GO:0008654 IEA
    response to bacterium GO:0009617 IEA
    lipid catabolic process GO:0016042 ISS
    membrane disassembly GO:0030397 ISS
    phosphatidylethanolamine acyl-chain remodeling GO:0036152 TAS
    ether lipid metabolic process GO:0046485 ISS
    N-acylphosphatidylethanolamine metabolic process GO:0070292 IBA
    N-acylphosphatidylethanolamine metabolic process GO:0070292 IDA
    lens fiber cell differentiation GO:0070306 ISS
    organelle disassembly GO:1903008 ISS
    regulation of adipose tissue development GO:1904177 ISS
Subcellular Localization
    nuclear envelope GO:0005635 ISS
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 ISS
    lysosome GO:0005764 ISS
    lysosomal membrane GO:0005765 IEA
    peroxisome GO:0005777 ISS
    peroxisomal membrane GO:0005778 IEA
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    mitochondrial membrane GO:0031966 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified PLA2G16 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PLA2G16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBQLN2 29978
Two-hybrid Homo sapiens
2 MIB2 142678
Affinity Capture-MS Homo sapiens
3 PRKCSH 5589
Affinity Capture-MS Homo sapiens
4 UBQLN1 29979
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 HRAS 3265
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 UBQLN4 56893
Two-hybrid Homo sapiens
7 PPP2R1A 5518
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 OCLN 100506658
Affinity Capture-MS Homo sapiens
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