Gene description for Prkag2
Gene name protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Gene symbol Prkag2
Other names/aliases 2410051C13Rik
AAKG
AAKG2
AI854673
H91620p
WPWS
Species Mus musculus
 Database cross references - Prkag2
ExoCarta ExoCarta_108099
Vesiclepedia VP_108099
Entrez Gene 108099
UniProt Q91WG5  
 Prkag2 identified in sEVs derived from the following tissue/cell type
Pancreatic cells 19351151    
 Gene ontology annotations for Prkag2
Molecular Function
    AMP-activated protein kinase activity GO:0004679 ISO
    AMP-activated protein kinase activity GO:0004679 TAS
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IEA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 ISO
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    cAMP-dependent protein kinase regulator activity GO:0008603 ISO
    phosphorylase kinase regulator activity GO:0008607 IEA
    phosphorylase kinase regulator activity GO:0008607 ISO
    AMP binding GO:0016208 IBA
    AMP binding GO:0016208 IEA
    AMP binding GO:0016208 ISO
    protein kinase regulator activity GO:0019887 IBA
    protein kinase regulator activity GO:0019887 ISO
    protein kinase regulator activity GO:0019887 ISS
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein kinase activator activity GO:0030295 IEA
    protein kinase activator activity GO:0030295 ISO
    ADP binding GO:0043531 IEA
    ADP binding GO:0043531 ISO
Biological Process
    glycogen metabolic process GO:0005977 IEA
    glycogen metabolic process GO:0005977 ISO
    regulation of glycolytic process GO:0006110 IBA
    regulation of glycolytic process GO:0006110 IEA
    regulation of glycolytic process GO:0006110 ISO
    negative regulation of protein kinase activity GO:0006469 ISO
    fatty acid biosynthetic process GO:0006633 IEA
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 ISO
    regulation of fatty acid metabolic process GO:0019217 IEA
    regulation of fatty acid metabolic process GO:0019217 ISO
    cellular response to nutrient levels GO:0031669 ISS
    intracellular signal transduction GO:0035556 IEA
    intracellular signal transduction GO:0035556 ISO
    cellular response to glucose starvation GO:0042149 IBA
    regulation of carbon utilization GO:0043609 IBA
    positive regulation of gluconeogenesis GO:0045722 IBA
    positive regulation of protein kinase activity GO:0045860 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    nucleotide-activated protein kinase complex GO:0031588 IBA
    nucleotide-activated protein kinase complex GO:0031588 ISO
    nucleotide-activated protein kinase complex GO:0031588 ISS
 Experiment description of studies that identified Prkag2 in sEVs
1
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Prkag2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Slc22a1  
Co-fractionation Mus musculus
3 Cers2  
Co-fractionation Mus musculus
4 PRKAA1 5562
Two-hybrid Homo sapiens
5 Slc22a21  
Co-fractionation Mus musculus
6 Sstr3  
Proximity Label-MS Mus musculus
7 Cyp2u1  
Co-fractionation Mus musculus
8 Cyp4f18  
Co-fractionation Mus musculus
9 Cyp2c54  
Co-fractionation Mus musculus
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