Gene description for DSN1
Gene name DSN1, MIS12 kinetochore complex component
Gene symbol DSN1
Other names/aliases C20orf172
KNL3
MIS13
dJ469A13.2
hKNL-3
Species Homo sapiens
 Database cross references - DSN1
ExoCarta ExoCarta_79980
Vesiclepedia VP_79980
Entrez Gene 79980
HGNC 16165
MIM 609175
UniProt Q9H410  
 DSN1 identified in sEVs derived from the following tissue/cell type
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for DSN1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    attachment of spindle microtubules to kinetochore GO:0008608 NAS
    skeletal muscle satellite cell proliferation GO:0014841 IEA
    cell division GO:0051301 IEA
Subcellular Localization
    MIS12/MIND type complex GO:0000444 IBA
    MIS12/MIND type complex GO:0000444 IDA
    MIS12/MIND type complex GO:0000444 NAS
    kinetochore GO:0000776 IDA
    kinetochore GO:0000776 NAS
    spindle pole GO:0000922 NAS
    outer kinetochore GO:0000940 IDA
    fibrillar center GO:0001650 IDA
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    nuclear body GO:0016604 IDA
    azurophil granule lumen GO:0035578 TAS
 Experiment description of studies that identified DSN1 in sEVs
1
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DSN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MIS12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HOMER3 9454
Negative Genetic Homo sapiens
3 NUF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CBX1 10951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 GNPDA2 132789
Negative Genetic Homo sapiens
6 BUB1  
Affinity Capture-MS Homo sapiens
7 RPS19BP1  
Affinity Capture-MS Homo sapiens
8 S100A6 6277
Affinity Capture-MS Homo sapiens
9 POGZ 23126
Affinity Capture-MS Homo sapiens
10 CENPC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NSL1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GAPDHS 26330
Affinity Capture-MS Homo sapiens
13 Ndc80  
Affinity Capture-MS Mus musculus
14 BUB1B  
Affinity Capture-MS Homo sapiens
15 ETFB 2109
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 COPA 1314
Affinity Capture-MS Homo sapiens
17 APP 351
Reconstituted Complex Homo sapiens
18 PMF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CENPA  
Proximity Label-MS Homo sapiens
20 PPP1CC 5501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 NDC80 10403
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 Dsn1  
Affinity Capture-MS Mus musculus
23 CHAF1A  
Affinity Capture-MS Homo sapiens
24 NAA11 84779
Affinity Capture-MS Homo sapiens
25 CASC5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 HGS 9146
Two-hybrid Homo sapiens
27 SRRM2 23524
Negative Genetic Homo sapiens
28 GUSBP5  
Affinity Capture-MS Homo sapiens
29 KRT78 196374
Affinity Capture-MS Homo sapiens
30 DNAJC7 7266
Affinity Capture-MS Homo sapiens
31 ZNF644  
Affinity Capture-MS Homo sapiens
32 Bub1b 12236
Affinity Capture-MS Mus musculus
33 ZWINT  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 NPAS1  
Affinity Capture-MS Homo sapiens
35 EXOC7 23265
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ICE2  
Affinity Capture-MS Homo sapiens
37 EXOC5 10640
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CCDC102A 92922
Affinity Capture-MS Homo sapiens
39 HTT 3064
Negative Genetic Homo sapiens
40 S100A2 6273
Affinity Capture-MS Homo sapiens
41 SCAI  
Affinity Capture-MS Homo sapiens
42 CTDP1 9150
Affinity Capture-MS Homo sapiens
43 LRIF1  
Affinity Capture-MS Homo sapiens
44 PURG  
Affinity Capture-MS Homo sapiens
45 CBX3 11335
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 BRCA2 675
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 UBR5 51366
Affinity Capture-MS Homo sapiens
48 MYH9 4627
Affinity Capture-MS Homo sapiens
49 CBX5 23468
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 EXOC6 54536
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PML 5371
Affinity Capture-MS Homo sapiens
52 Bub1  
Affinity Capture-MS Mus musculus
53 AIMP2 7965
Two-hybrid Homo sapiens
54 RNF40 9810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 S100P 6286
Affinity Capture-MS Homo sapiens
56 Zwint  
Affinity Capture-MS Mus musculus
57 NR3C1 2908
Proximity Label-MS Homo sapiens
58 GYPA  
Affinity Capture-MS Homo sapiens
59 WIZ 58525
Affinity Capture-MS Homo sapiens
60 SPC24 147841
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 SGOL1  
Affinity Capture-MS Homo sapiens
62 TTC3 7267
Affinity Capture-MS Homo sapiens
63 ADNP 23394
Affinity Capture-MS Homo sapiens
64 DUSP16  
Affinity Capture-MS Homo sapiens
65 TP73  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 RBBP4 5928
Affinity Capture-MS Homo sapiens
67 NXF1 10482
Affinity Capture-RNA Homo sapiens
68 MLF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 Mis12  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
70 EHMT2 10919
Affinity Capture-MS Homo sapiens
71 HSPA4 3308
Affinity Capture-MS Homo sapiens
72 TMEM101  
Negative Genetic Homo sapiens
73 FHL2 2274
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 KIF2B 84643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 SUGT1 10910
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
76 SALL2  
Affinity Capture-MS Homo sapiens
77 BCAS2 10286
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 WDFY2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 CHAMP1  
Affinity Capture-MS Homo sapiens
80 ADNP2  
Affinity Capture-MS Homo sapiens
81 CHD4 1108
Affinity Capture-MS Homo sapiens
82 RNF20 56254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
84 SALL1 6299
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 PCNA 5111
Affinity Capture-MS Homo sapiens
86 SPC25 57405
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 Casc5  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
88 PYGM 5837
Affinity Capture-MS Homo sapiens
89 MOV10 4343
Affinity Capture-RNA Homo sapiens
90 PCGF6  
Affinity Capture-MS Homo sapiens
91 POLR3B 55703
Affinity Capture-MS Homo sapiens
92 TPST2 8459
Affinity Capture-MS Homo sapiens
93 EHMT1  
Affinity Capture-MS Homo sapiens
94 CHAF1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 YBEY  
Affinity Capture-MS Homo sapiens
96 SKA2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
97 PIK3R3 8503
Two-hybrid Homo sapiens
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