Gene description for RBP1
Gene name retinol binding protein 1, cellular
Gene symbol RBP1
Other names/aliases CRABP-I
CRBP
CRBP1
CRBPI
RBPC
Species Homo sapiens
 Database cross references - RBP1
ExoCarta ExoCarta_5947
Vesiclepedia VP_5947
Entrez Gene 5947
HGNC 9919
MIM 180260
UniProt P09455  
 RBP1 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for RBP1
Molecular Function
    retinoid binding GO:0005501 TAS
    fatty acid binding GO:0005504 IBA
    protein binding GO:0005515 IPI
    retinal binding GO:0016918 IEA
    all-trans-retinol binding GO:1904768 IBA
    all-trans-retinol binding GO:1904768 IDA
Biological Process
    retinoic acid biosynthetic process GO:0002138 IEA
    vitamin A metabolic process GO:0006776 IMP
    fatty acid transport GO:0015908 IBA
    lipid homeostasis GO:0055088 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    lipid droplet GO:0005811 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified RBP1 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for RBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZRANB1 54764
Affinity Capture-MS Homo sapiens
2 UBE2J2 118424
Two-hybrid Homo sapiens
3 PTMA 5757
Cross-Linking-MS (XL-MS) Homo sapiens
4 PRNP 5621
Affinity Capture-MS Homo sapiens
5 IKBKG 8517
Two-hybrid Homo sapiens
6 HIST3H2BB 128312
Affinity Capture-MS Homo sapiens
7 SAP30 8819
Affinity Capture-Western Homo sapiens
8 SFR1 119392
Affinity Capture-MS Homo sapiens
9 SIRT1  
Affinity Capture-Western Homo sapiens
10 SOX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CUL4B 8450
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 ING1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 SLC4A5  
Affinity Capture-MS Homo sapiens
14 FAM24B  
Affinity Capture-MS Homo sapiens
15 LRP2 4036
Reconstituted Complex Homo sapiens
16 INO80  
Affinity Capture-MS Homo sapiens
17 OR52N4  
Affinity Capture-MS Homo sapiens
18 SIRT2 22933
Affinity Capture-Western Homo sapiens
19 DDB1 1642
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 OR2A4 79541
Affinity Capture-MS Homo sapiens
21 EEF2K  
Affinity Capture-MS Homo sapiens
22 UCHL5 51377
Affinity Capture-MS Homo sapiens
23 BRMS1L  
Affinity Capture-MS Homo sapiens
24 TNFRSF10D 8793
Two-hybrid Homo sapiens
25 ARPC1A 10552
Affinity Capture-MS Homo sapiens
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