Gene description for PRKG1
Gene name protein kinase, cGMP-dependent, type I
Gene symbol PRKG1
Other names/aliases 1
AAT8
PKG
PRKG1B
PRKGR1B
cGK
cGK 1
cGK1
cGKI
cGKI-BETA
cGKI-alpha
Species Homo sapiens
 Database cross references - PRKG1
ExoCarta ExoCarta_5592
Vesiclepedia VP_5592
Entrez Gene 5592
HGNC 9414
MIM 176894
UniProt Q13976  
 PRKG1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PRKG1
Molecular Function
    protein kinase activity GO:0004672 IMP
    cGMP-dependent protein kinase activity GO:0004692 IDA
    cGMP-dependent protein kinase activity GO:0004692 TAS
    calcium channel regulator activity GO:0005246 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    cGMP binding GO:0030553 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 IPI
    mitogen-activated protein kinase p38 binding GO:0048273 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    neuron migration GO:0001764 IEA
    protein phosphorylation GO:0006468 IDA
    signal transduction GO:0007165 IBA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    spermatid development GO:0007286 IEA
    negative regulation of inositol phosphate biosynthetic process GO:0010920 IEA
    negative regulation of glutamate secretion GO:0014050 IEA
    dendrite development GO:0016358 IEA
    cGMP-mediated signaling GO:0019934 IEA
    cerebellum development GO:0021549 IEA
    actin cytoskeleton organization GO:0030036 TAS
    forebrain development GO:0030900 IEA
    positive regulation of circadian rhythm GO:0042753 IEA
    regulation of GTPase activity GO:0043087 IMP
    collateral sprouting GO:0048668 IEA
    relaxation of vascular associated smooth muscle GO:0060087 IEA
    cell growth involved in cardiac muscle cell development GO:0061049 IEA
    negative regulation of platelet aggregation GO:0090331 IMP
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 IDA
    negative regulation of vascular associated smooth muscle cell migration GO:1904753 IDA
    regulation of testosterone biosynthetic process GO:2000224 IEA
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    nucleoplasm GO:0005654 IEA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    sarcolemma GO:0042383 IEA
 Experiment description of studies that identified PRKG1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PRKG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP1R12B 4660
Two-hybrid Homo sapiens
2 KIF2A 3796
Affinity Capture-MS Homo sapiens
3 GAGE1  
Affinity Capture-MS Homo sapiens
4 ARHGEF15  
Two-hybrid Homo sapiens
5 WDR77 79084
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
6 GTF2I 2969
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 DDX31  
Affinity Capture-MS Homo sapiens
8 ZKSCAN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RGS2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 RBAK  
Two-hybrid Homo sapiens
11 HDAC1 3065
Biochemical Activity Homo sapiens
12 PTK2 5747
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 OAS2 4939
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 VHL  
Two-hybrid Homo sapiens
15 TRIM54  
Two-hybrid Homo sapiens
16 LMO2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 ZNF646  
Two-hybrid Homo sapiens
18 CORO2B 10391
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MEOX2  
Two-hybrid Homo sapiens
20 PPP1R12A 4659
Reconstituted Complex Homo sapiens
21 MKRN2 23609
Affinity Capture-RNA Homo sapiens
22 HECW2  
Affinity Capture-MS Homo sapiens
23 TRIM39-RPP21  
Two-hybrid Homo sapiens
24 NPR1 4881
Two-hybrid Homo sapiens
25 ITPR1 3708
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
26 PSMC5 5705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 RAF1 5894
Affinity Capture-Western Homo sapiens
28 AR 367
Biochemical Activity Homo sapiens
29 SELL 6402
Biochemical Activity Homo sapiens
30 FAM124A  
Two-hybrid Homo sapiens
31 TNNT1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
32 MRVI1 10335
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
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