Gene description for LETM1
Gene name leucine zipper-EF-hand containing transmembrane protein 1
Gene symbol LETM1
Other names/aliases -
Species Homo sapiens
 Database cross references - LETM1
ExoCarta ExoCarta_3954
Vesiclepedia VP_3954
Entrez Gene 3954
HGNC 6556
MIM 604407
UniProt O95202  
 LETM1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Prostate cancer cells 25844599    
T lymphocytes 34108659    
 Gene ontology annotations for LETM1
Molecular Function
    calcium ion binding GO:0005509 TAS
    protein binding GO:0005515 IPI
    calcium:proton antiporter activity GO:0015369 IBA
    calcium:proton antiporter activity GO:0015369 IDA
    ribosome binding GO:0043022 IEA
Biological Process
    calcium ion transport GO:0006816 IMP
    mitochondrial calcium ion transmembrane transport GO:0006851 IBA
    mitochondrial calcium ion transmembrane transport GO:0006851 IDA
    mitochondrial calcium ion transmembrane transport GO:0006851 IMP
    mitochondrion organization GO:0007005 IBA
    inner mitochondrial membrane organization GO:0007007 IMP
    protein hexamerization GO:0034214 ISS
    cristae formation GO:0042407 IMP
    protein homooligomerization GO:0051260 ISS
    mitochondrial calcium ion homeostasis GO:0051560 IDA
    negative regulation of mitochondrial calcium ion concentration GO:0051562 IMP
    calcium export from the mitochondrion GO:0099093 IDA
    calcium export from the mitochondrion GO:0099093 IMP
    mitochondrial potassium ion transmembrane transport GO:0140141 IMP
    regulation of cellular hyperosmotic salinity response GO:1900069 IMP
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 TAS
    mitochondrial inner membrane GO:0005743 IDA
 Experiment description of studies that identified LETM1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
18
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
27
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for LETM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSD3B2 3284
Affinity Capture-MS Homo sapiens
2 TBK1 29110
Affinity Capture-MS Homo sapiens
3 SLIRP 81892
Proximity Label-MS Homo sapiens
4 TMEM183A  
Affinity Capture-MS Homo sapiens
5 CLIP1 6249
Affinity Capture-MS Homo sapiens
6 DNAJA3 9093
Proximity Label-MS Homo sapiens
7 MYLK4  
Affinity Capture-MS Homo sapiens
8 RIN3  
Affinity Capture-MS Homo sapiens
9 ACOT9 23597
Co-fractionation Homo sapiens
10 RCL1 10171
Affinity Capture-MS Homo sapiens
11 PCDHGB4 8641
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TRUB2  
Proximity Label-MS Homo sapiens
13 MTG2  
Proximity Label-MS Homo sapiens
14 NGRN  
Proximity Label-MS Homo sapiens
15 CHCHD1  
Proximity Label-MS Homo sapiens
16 NTNG1  
Affinity Capture-MS Homo sapiens
17 RPUSD4 84881
Proximity Label-MS Homo sapiens
18 ASPH 444
Co-fractionation Homo sapiens
19 DNAJC15  
Proximity Label-MS Homo sapiens
20 MTRF1  
Proximity Label-MS Homo sapiens
21 GFM2 84340
Proximity Label-MS Homo sapiens
22 CCDC90B  
Proximity Label-MS Homo sapiens
23 DNAJB6 10049
Affinity Capture-MS Homo sapiens
24 LRPPRC 10128
Proximity Label-MS Homo sapiens
25 KIAA1429 25962
Affinity Capture-MS Homo sapiens
26 HEATR3 55027
Affinity Capture-MS Homo sapiens
27 MCU 90550
Proximity Label-MS Homo sapiens
28 B3GNT2 10678
Affinity Capture-MS Homo sapiens
29 CRYZ 1429
Proximity Label-MS Homo sapiens
30 TFAM 7019
Proximity Label-MS Homo sapiens
31 PMPCA 23203
Proximity Label-MS Homo sapiens
32 COQ9  
Affinity Capture-MS Homo sapiens
33 C8orf82  
Proximity Label-MS Homo sapiens
34 PTPMT1 114971
Affinity Capture-MS Homo sapiens
35 CYB5R1 51706
Co-fractionation Homo sapiens
36 TIMMDC1  
Affinity Capture-MS Homo sapiens
37 HINT2 84681
Proximity Label-MS Homo sapiens
38 TREML2 79865
Affinity Capture-MS Homo sapiens
39 RER1 11079
Affinity Capture-MS Homo sapiens
40 DNAJB1 3337
Affinity Capture-MS Homo sapiens
41 MRPS26 64949
Proximity Label-MS Homo sapiens
42 SURF1  
Proximity Label-MS Homo sapiens
43 C3orf52  
Affinity Capture-MS Homo sapiens
44 PPM1A 5494
Affinity Capture-MS Homo sapiens
45 ITFG3 83986
Affinity Capture-MS Homo sapiens
46 HYPM  
Affinity Capture-MS Homo sapiens
47 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
48 C10orf107  
Affinity Capture-MS Homo sapiens
49 MLC1 23209
Affinity Capture-MS Homo sapiens
50 WIF1 11197
Affinity Capture-MS Homo sapiens
51 FSD2 123722
Affinity Capture-MS Homo sapiens
52 PMPCB 9512
Proximity Label-MS Homo sapiens
53 TACO1  
Proximity Label-MS Homo sapiens
54 FASTKD2  
Proximity Label-MS Homo sapiens
55 VWA8 23078
Proximity Label-MS Homo sapiens
56 MTERF3  
Proximity Label-MS Homo sapiens
57 KIF14 9928
Affinity Capture-MS Homo sapiens
58 PCNA 5111
Cross-Linking-MS (XL-MS) Homo sapiens
59 MDH2 4191
Proximity Label-MS Homo sapiens
60 APOO 79135
Proximity Label-MS Homo sapiens
61 ATP2A2 488
Co-fractionation Homo sapiens
62 DNAL4  
Affinity Capture-MS Homo sapiens
63 C6orf203  
Proximity Label-MS Homo sapiens
64 COX15 1355
Proximity Label-MS Homo sapiens
65 FASTKD5  
Proximity Label-MS Homo sapiens
66 PEX3 8504
Proximity Label-MS Homo sapiens
67 RTN1 6252
Affinity Capture-MS Homo sapiens
68 OPTN 10133
Affinity Capture-MS Homo sapiens
69 HADHA 3030
Co-fractionation Homo sapiens
70 TUFM 7284
Proximity Label-MS Homo sapiens
71 HSCB 150274
Proximity Label-MS Homo sapiens
72 SLC25A20 788
Co-fractionation Homo sapiens
73 CDKAL1  
Affinity Capture-MS Homo sapiens
74 MYH9 4627
Affinity Capture-MS Homo sapiens
75 NTRK1 4914
Affinity Capture-MS Homo sapiens
76 KRTAP6-2  
Two-hybrid Homo sapiens
77 CCDC109B 55013
Proximity Label-MS Homo sapiens
78 EXD2  
Proximity Label-MS Homo sapiens
79 SYNCRIP 10492
Co-fractionation Homo sapiens
80 TBRG4 9238
Proximity Label-MS Homo sapiens
81 RPL36 25873
Cross-Linking-MS (XL-MS) Homo sapiens
82 PREB 10113
Co-fractionation Homo sapiens
83 SNRNP27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 MTFMT  
Proximity Label-MS Homo sapiens
85 MRPL11 65003
Proximity Label-MS Homo sapiens
86 MTRF1L  
Proximity Label-MS Homo sapiens
87 RABIF  
Affinity Capture-MS Homo sapiens
88 AGPS 8540
Co-fractionation Homo sapiens
89 LATS2 26524
Affinity Capture-MS Homo sapiens
90 LYPD4  
Affinity Capture-MS Homo sapiens
91 TMEM70  
Proximity Label-MS Homo sapiens
92 CARTPT  
Affinity Capture-MS Homo sapiens
93 FGFR1OP  
Affinity Capture-MS Homo sapiens
94 PIPSL 266971
Affinity Capture-MS Homo sapiens
95 COX4I1 1327
Proximity Label-MS Homo sapiens
96 GSX1  
Affinity Capture-MS Homo sapiens
97 APOE 348
Co-fractionation Homo sapiens
98 C15orf48  
Affinity Capture-MS Homo sapiens
99 OGT 8473
Reconstituted Complex Homo sapiens
100 OTC 5009
Proximity Label-MS Homo sapiens
101 PSMA3 5684
Two-hybrid Homo sapiens
102 MRRF  
Proximity Label-MS Homo sapiens
103 AIFM1 9131
Proximity Label-MS Homo sapiens
104 MRPS12  
Proximity Label-MS Homo sapiens
105 RAB7A 7879
Co-fractionation Homo sapiens
106 MTIF2 4528
Proximity Label-MS Homo sapiens
107 C9orf78 51759
Affinity Capture-MS Homo sapiens
108 MRPS11  
Affinity Capture-MS Homo sapiens
109 MTIF3  
Proximity Label-MS Homo sapiens
110 CRYAA 1409
Affinity Capture-MS Homo sapiens
111 TUBB4B 10383
Affinity Capture-MS Homo sapiens
112 MEX3A  
Affinity Capture-RNA Homo sapiens
113 CENPM  
Affinity Capture-MS Homo sapiens
114 ASS1 445
Affinity Capture-MS Homo sapiens
115 SYT6  
Affinity Capture-MS Homo sapiens
116 RMND1 55005
Proximity Label-MS Homo sapiens
117 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 OSGEP 55644
Affinity Capture-MS Homo sapiens
119 ARF6 382
Proximity Label-MS Homo sapiens
120 C17orf80 55028
Proximity Label-MS Homo sapiens
121 C12orf65  
Proximity Label-MS Homo sapiens
122 MAPK6  
Affinity Capture-MS Homo sapiens
123 MTG1  
Proximity Label-MS Homo sapiens
124 NDUFA4 4697
Affinity Capture-MS Homo sapiens
125 LRRC14B  
Affinity Capture-MS Homo sapiens
126 USP47 55031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 EGFR 1956
Negative Genetic Homo sapiens
128 RPL35A 6165
Affinity Capture-MS Homo sapiens
129 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
130 ACAD9 28976
Proximity Label-MS Homo sapiens
131 DDB1 1642
Affinity Capture-MS Homo sapiens
132 ICE2  
Affinity Capture-MS Homo sapiens
133 CYB5R3 1727
Co-fractionation Homo sapiens
134 ATP5C1 509
Co-fractionation Homo sapiens
135 HSPA9 3313
Proximity Label-MS Homo sapiens
136 METTL15  
Proximity Label-MS Homo sapiens
137 RTN4 57142
Affinity Capture-MS Homo sapiens
138 AKAP1 8165
Proximity Label-MS Homo sapiens
139 MCUR1 63933
Proximity Label-MS Homo sapiens
140 SCCPDH 51097
Co-fractionation Homo sapiens
141 PHGDH 26227
Affinity Capture-MS Homo sapiens
142 FTL 2512
Affinity Capture-MS Homo sapiens
143 CLPP 8192
Proximity Label-MS Homo sapiens
144 DNAJC28  
Proximity Label-MS Homo sapiens
145 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
146 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
147 PHB2 11331
Proximity Label-MS Homo sapiens
148 TSFM 10102
Proximity Label-MS Homo sapiens
149 AUH 549
Proximity Label-MS Homo sapiens
150 RAB5B 5869
Co-fractionation Homo sapiens
151 ILF3 3609
Co-fractionation Homo sapiens
152 DNAJC19 131118
Proximity Label-MS Homo sapiens
153 RNF19B  
Affinity Capture-MS Homo sapiens
154 ICT1 3396
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
155 PEX19 5824
Affinity Capture-MS Homo sapiens
156 LONP1 9361
Proximity Label-MS Homo sapiens
157 PDHA1 5160
Proximity Label-MS Homo sapiens
158 FGB 2244
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 COX8A  
Proximity Label-MS Homo sapiens
160 C21orf33  
Proximity Label-MS Homo sapiens
161 SSBP1 6742
Proximity Label-MS Homo sapiens
162 SFXN1 94081
Proximity Label-MS Homo sapiens
163 FASTKD3  
Proximity Label-MS Homo sapiens
164 GFM1 85476
Proximity Label-MS Homo sapiens
165 AARS2  
Proximity Label-MS Homo sapiens
166 TEFM  
Proximity Label-MS Homo sapiens
167 MND1  
Affinity Capture-MS Homo sapiens
168 C19orf52  
Proximity Label-MS Homo sapiens
169 SLC25A51  
Proximity Label-MS Homo sapiens
170 RPUSD3  
Proximity Label-MS Homo sapiens
171 EP300 2033
Affinity Capture-MS Homo sapiens
172 GRSF1 2926
Proximity Label-MS Homo sapiens
173 C1QBP 708
Proximity Label-MS Homo sapiens
174 SERBP1 26135
Affinity Capture-MS Homo sapiens
175 KRAS 3845
Negative Genetic Homo sapiens
176 UBE2Q2 92912
Affinity Capture-MS Homo sapiens
177 METTL17  
Proximity Label-MS Homo sapiens
178 OR2T10  
Affinity Capture-MS Homo sapiens
179 RPA3 6119
Proximity Label-MS Homo sapiens
180 OGDH 4967
Co-fractionation Homo sapiens
181 DDX28  
Proximity Label-MS Homo sapiens
182 SNAP29 9342
Affinity Capture-MS Homo sapiens
183 C12orf57 113246
Co-fractionation Homo sapiens
View the network image/svg+xml



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