Gene description for HRAS
Gene name Harvey rat sarcoma viral oncogene homolog
Gene symbol HRAS
Other names/aliases C-BAS/HAS
C-H-RAS
C-HA-RAS1
CTLO
H-RASIDX
HAMSV
HRAS1
RASH1
p21ras
Species Homo sapiens
 Database cross references - HRAS
ExoCarta ExoCarta_3265
Vesiclepedia VP_3265
Entrez Gene 3265
HGNC 5173
MIM 190020
UniProt P01112  
 HRAS identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
Urine 15326289    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for HRAS
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 IMP
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IMP
    GDP binding GO:0019003 IBA
    GDP binding GO:0019003 IMP
    protein-membrane adaptor activity GO:0043495 IEA
    phospholipase C activator activity GO:0160185 IDA
Biological Process
    MAPK cascade GO:0000165 TAS
    positive regulation of protein phosphorylation GO:0001934 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    endocytosis GO:0006897 IEA
    chemotaxis GO:0006935 TAS
    signal transduction GO:0007165 NAS
    cell surface receptor signaling pathway GO:0007166 TAS
    Ras protein signal transduction GO:0007265 IBA
    Ras protein signal transduction GO:0007265 IDA
    positive regulation of cell population proliferation GO:0008284 IDA
    negative regulation of cell population proliferation GO:0008285 IDA
    insulin receptor signaling pathway GO:0008286 IEA
    animal organ morphogenesis GO:0009887 TAS
    negative regulation of gene expression GO:0010629 IDA
    Schwann cell development GO:0014044 IEA
    positive regulation of cell migration GO:0030335 IDA
    positive regulation of type II interferon production GO:0032729 IEA
    regulation of actin cytoskeleton organization GO:0032956 IDA
    negative regulation of GTPase activity GO:0034260 IDA
    T-helper 1 type immune response GO:0042088 IEA
    regulation of cell population proliferation GO:0042127 IDA
    myelination GO:0042552 IEA
    defense response to protozoan GO:0042832 IEA
    positive regulation of MAP kinase activity GO:0043406 IDA
    positive regulation of MAPK cascade GO:0043410 IDA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    positive regulation of GTPase activity GO:0043547 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of JNK cascade GO:0046330 IDA
    fibroblast proliferation GO:0048144 IEA
    positive regulation of fibroblast proliferation GO:0048146 IEA
    regulation of long-term neuronal synaptic plasticity GO:0048169 IEA
    positive regulation of epithelial cell proliferation GO:0050679 IMP
    T cell receptor signaling pathway GO:0050852 IEA
    neuron apoptotic process GO:0051402 IEA
    regulation of cell cycle GO:0051726 IDA
    adipose tissue development GO:0060612 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    cellular response to gamma radiation GO:0071480 IDA
    positive regulation of wound healing GO:0090303 IDA
    positive regulation of protein targeting to membrane GO:0090314 IMP
    cellular senescence GO:0090398 IBA
    cellular senescence GO:0090398 IDA
    oncogene-induced cell senescence GO:0090402 IEA
    intrinsic apoptotic signaling pathway GO:0097193 IEA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IDA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IMP
    positive regulation of ruffle assembly GO:1900029 IDA
    positive regulation of miRNA metabolic process GO:2000630 IDA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    GTPase complex GO:1905360 IPI
 Experiment description of studies that identified HRAS in sEVs
1
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
14
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HRAS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GPSM2  
Affinity Capture-Western Homo sapiens
2 ZDHHC9  
Biochemical Activity Homo sapiens
3 HDAC4  
Affinity Capture-MS Homo sapiens
4 ABL2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 RGL4  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
6 SNRPE 6635
Two-hybrid Homo sapiens
7 RASGRF1  
Two-hybrid Homo sapiens
8 PMS1  
Affinity Capture-MS Homo sapiens
9 DHCR24 1718
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ANLN 54443
Affinity Capture-MS Homo sapiens
11 KRT17 3872
Reconstituted Complex Homo sapiens
12 ANKRD34B  
Reconstituted Complex Homo sapiens
13 LGALS1 3956
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 PDE4D  
Reconstituted Complex Homo sapiens
15 UBE3B 89910
Reconstituted Complex Homo sapiens
16 ANAPC1 64682
Reconstituted Complex Homo sapiens
17 TTC21A  
Reconstituted Complex Homo sapiens
18 MRPL49 740
Affinity Capture-MS Homo sapiens
19 RABGGTB 5876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 IL20  
Affinity Capture-MS Homo sapiens
21 OTUD3 23252
Affinity Capture-MS Homo sapiens
22 PIK3CD 5293
Two-hybrid Homo sapiens
23 RGL1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
24 DUSP12 11266
Affinity Capture-MS Homo sapiens
25 RHEB 6009
Affinity Capture-Western Homo sapiens
26 UXS1 80146
Affinity Capture-MS Homo sapiens
27 WDR76  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
28 ZBTB10 65986
Reconstituted Complex Homo sapiens
29 MLLT4 4301
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 PLAU 5328
Phenotypic Suppression Homo sapiens
31 DPP9 91039
Reconstituted Complex Homo sapiens
32 DGKE  
Reconstituted Complex Homo sapiens
33 ABR 29
Reconstituted Complex Homo sapiens
34 TLR6  
Reconstituted Complex Homo sapiens
35 PIK3CG  
Co-crystal Structure Homo sapiens
36 TIAM1  
Affinity Capture-Western Homo sapiens
37 CUL4A 8451
Affinity Capture-Western Homo sapiens
38 MAPK10 5602
Reconstituted Complex Homo sapiens
39 RASA4 10156
Reconstituted Complex Homo sapiens
40 RASSF5 83593
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
41 HINT3 135114
Affinity Capture-MS Homo sapiens
42 MAP3K6  
Reconstituted Complex Homo sapiens
43 SNX14  
Reconstituted Complex Homo sapiens
44 BTRC 8945
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
45 ERBB2 2064
Reconstituted Complex Homo sapiens
46 RALGDS  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
47 CBL 867
Affinity Capture-Western Homo sapiens
48 PRNP 5621
Affinity Capture-MS Homo sapiens
49 ANKRD16  
Reconstituted Complex Homo sapiens
50 RABGEF1 27342
Biochemical Activity Homo sapiens
51 RAB5A 5868
Affinity Capture-Western Homo sapiens
52 ITGB3 3690
Reconstituted Complex Homo sapiens
53 CYP2S1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 UBE4B 10277
Reconstituted Complex Homo sapiens
55 RSPO3  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
56 RASA2 5922
Reconstituted Complex Homo sapiens
57 IGFBP5 3488
Affinity Capture-MS Homo sapiens
58 PLA2G4B  
Reconstituted Complex Homo sapiens
59 RNF115  
Two-hybrid Homo sapiens
60 DEFB104A  
Affinity Capture-MS Homo sapiens
61 ARHGEF1 9138
Biochemical Activity Homo sapiens
62 CERS2 29956
Affinity Capture-MS Homo sapiens
63 GJB7  
Affinity Capture-MS Homo sapiens
64 APBB1IP 54518
Reconstituted Complex Homo sapiens
65 NTRK3 4916
Affinity Capture-MS Homo sapiens
66 ZHX2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
67 HECTD1 25831
Reconstituted Complex Homo sapiens
68 TIGD5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 RIN1 9610
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
70 CENPM  
Affinity Capture-MS Homo sapiens
71 GPSM3  
Reconstituted Complex Homo sapiens
72 NRAS 4893
Reconstituted Complex Homo sapiens
73 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
74 BRAF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
75 FYN 2534
Reconstituted Complex Homo sapiens
76 BRAP  
Affinity Capture-Western Homo sapiens
77 MAPK8 5599
Phenotypic Suppression Homo sapiens
78 LATS2 26524
Affinity Capture-Western Homo sapiens
79 ACTG1 71
Reconstituted Complex Homo sapiens
80 PLCXD2  
Affinity Capture-MS Homo sapiens
81 ARFGAP1 55738
Reconstituted Complex Homo sapiens
82 TMEM185A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 DUSP22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 HSPA1L 3305
Reconstituted Complex Homo sapiens
85 S1PR1 1901
Affinity Capture-MS Homo sapiens
86 RAP1GDS1 5910
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
87 FAM129B 64855
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
88 DESI1  
Affinity Capture-MS Homo sapiens
89 RABGGTA 5875
Affinity Capture-MS Homo sapiens
90 HBG2 3048
Affinity Capture-MS Homo sapiens
91 ARHGAP10 79658
Reconstituted Complex Homo sapiens
92 cno  
Reconstituted Complex Drosophila melanogaster
93 SDC1 6382
Affinity Capture-MS Homo sapiens
94 KIF20A 10112
Affinity Capture-MS Homo sapiens
95 TPR 7175
Co-fractionation Homo sapiens
96 IKZF3  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
97 HOXB5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 ERBB2IP 55914
Affinity Capture-Western Homo sapiens
99 SCML1  
Affinity Capture-MS Homo sapiens
100 IL22RA1 58985
Negative Genetic Homo sapiens
101 QPCT 25797
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
102 DAB2IP 153090
Affinity Capture-Western Homo sapiens
103 SLC9A3R2 9351
Co-fractionation Homo sapiens
104 RASIP1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
105 RASSF1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
106 HDHD2 84064
Affinity Capture-MS Homo sapiens
107 PCDHGC3 5098
Affinity Capture-MS Homo sapiens
108 PI4K2A 55361
Reconstituted Complex Homo sapiens
109 FAM195A  
Affinity Capture-MS Homo sapiens
110 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 CYP51A1 1595
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 DGKZ  
Affinity Capture-Western Homo sapiens
113 ARHGAP29 9411
Reconstituted Complex Homo sapiens
114 IL1RL1 9173
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
115 SNX17 9784
Reconstituted Complex Homo sapiens
116 PLA2G16 11145
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
117 PIK3CA 5290
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
118 DUSP9  
Affinity Capture-MS Homo sapiens
119 RAF1 5894
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
120 KRTAP6-1  
Affinity Capture-MS Homo sapiens
121 KRAS 3845
Reconstituted Complex Homo sapiens
122 PLCH2  
Reconstituted Complex Homo sapiens
123 DHRS3 9249
Affinity Capture-MS Homo sapiens
124 ANKRD23 200539
Reconstituted Complex Homo sapiens
125 MRPL38  
Affinity Capture-MS Homo sapiens
126 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
127 MLLT4  
Reconstituted Complex Bos taurus
128 RASA1 5921
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
129 PSMB2 5690
Co-fractionation Homo sapiens
130 KRT18 3875
Two-hybrid Homo sapiens
131 MTHFD2 10797
Affinity Capture-MS Homo sapiens
132 ARHGEF18 23370
Reconstituted Complex Homo sapiens
133 DUS4L  
Affinity Capture-MS Homo sapiens
134 TTC1 7265
Affinity Capture-Western Homo sapiens
135 SGK1  
Affinity Capture-MS Homo sapiens
136 SPRY2 10253
Affinity Capture-Western Homo sapiens
137 RGL2 5863
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
138 USP29  
Reconstituted Complex Homo sapiens
139 TP53 7157
Affinity Capture-Western Homo sapiens
140 PIGO  
Affinity Capture-MS Homo sapiens
141 TTC28 23331
Reconstituted Complex Homo sapiens
142 DEAF1 10522
Two-hybrid Homo sapiens
143 STK38 11329
Reconstituted Complex Homo sapiens
144 CD27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 CCR1  
Affinity Capture-MS Homo sapiens
146 SOS1 6654
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
147 INSR 3643
Biochemical Activity Homo sapiens
148 PHF19  
Affinity Capture-MS Homo sapiens
149 ZDHHC23  
Affinity Capture-MS Homo sapiens
150 MAP2K1 5604
Affinity Capture-Western Homo sapiens
151 PDE6D 5147
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
152 FAM213B 127281
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
153 NF1 4763
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
154 BTBD2  
Affinity Capture-MS Homo sapiens
155 MED21  
Affinity Capture-MS Homo sapiens
156 PPP1R13B  
Affinity Capture-Western Homo sapiens
157 MOV10 4343
Affinity Capture-RNA Homo sapiens
158 MGST3 4259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 SHOC2 8036
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
160 OR52I1  
Affinity Capture-MS Homo sapiens
161 LZTR1 8216
Reconstituted Complex Homo sapiens
162 NEDD8 4738
Co-fractionation Homo sapiens
163 ANKRD11 29123
Reconstituted Complex Homo sapiens
164 STC2 8614
Affinity Capture-MS Homo sapiens
165 RAB5C 5878
Reconstituted Complex Homo sapiens
166 C9orf72  
Affinity Capture-MS Homo sapiens
167 USP42  
Reconstituted Complex Homo sapiens
168 VDAC3 7419
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
169 HSPA12A 259217
Reconstituted Complex Homo sapiens
170 OR2T10  
Affinity Capture-MS Homo sapiens
171 C1QL4  
Affinity Capture-MS Homo sapiens
172 ATG3 64422
Affinity Capture-MS Homo sapiens
173 EIF3L 51386
Reconstituted Complex Homo sapiens
174 CDC123 8872
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which HRAS is involved
PathwayEvidenceSource
Activated NTRK2 signals through FRS2 and FRS3 IEA Reactome
Activated NTRK2 signals through RAS IEA Reactome
Activated NTRK3 signals through RAS IEA Reactome
Activation of NMDA receptors and postsynaptic events IEA Reactome
Activation of RAS in B cells TAS Reactome
Adaptive Immune System TAS Reactome
Axon guidance TAS Reactome
C-type lectin receptors (CLRs) TAS Reactome
CD209 (DC-SIGN) signaling TAS Reactome
Cell surface interactions at the vascular wall TAS Reactome
Constitutive Signaling by EGFRvIII TAS Reactome
Constitutive Signaling by Ligand-Responsive EGFR Cancer Variants TAS Reactome
Constitutive Signaling by Overexpressed ERBB2 TAS Reactome
CREB1 phosphorylation through NMDA receptor-mediated activation of RAS signaling IEA Reactome
Cytokine Signaling in Immune system TAS Reactome
DAP12 interactions TAS Reactome
DAP12 signaling TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Downstream signal transduction TAS Reactome
Downstream signaling events of B Cell Receptor (BCR) TAS Reactome
Downstream signaling of activated FGFR1 TAS Reactome
Downstream signaling of activated FGFR2 TAS Reactome
Downstream signaling of activated FGFR3 TAS Reactome
Downstream signaling of activated FGFR4 TAS Reactome
EGFR Transactivation by Gastrin TAS Reactome
EPH-Ephrin signaling TAS Reactome
EPHB-mediated forward signaling TAS Reactome
Erythropoietin activates RAS IEA Reactome
ESR-mediated signaling TAS Reactome
Estrogen-stimulated signaling through PRKCZ TAS Reactome
Extra-nuclear estrogen signaling TAS Reactome
Fc epsilon receptor (FCERI) signaling TAS Reactome
FCERI mediated MAPK activation TAS Reactome
FLT3 Signaling TAS Reactome
FLT3 signaling in disease TAS Reactome
FRS-mediated FGFR1 signaling TAS Reactome
FRS-mediated FGFR2 signaling TAS Reactome
FRS-mediated FGFR3 signaling TAS Reactome
FRS-mediated FGFR4 signaling TAS Reactome
G alpha (q) signalling events TAS Reactome
Gastrin-CREB signalling pathway via PKC and MAPK TAS Reactome
GPCR downstream signalling TAS Reactome
GRB2 events in EGFR signaling TAS Reactome
GRB2 events in ERBB2 signaling TAS Reactome
GRB2 events in ERBB2 signaling IEA Reactome
Hemostasis TAS Reactome
IGF1R signaling cascade IEA Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Insulin receptor signalling cascade IEA Reactome
IRS-mediated signalling IEA Reactome
IRS-related events triggered by IGF1R IEA Reactome
MAP2K and MAPK activation TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
MET activates RAS signaling TAS Reactome
NCAM signaling for neurite out-growth TAS Reactome
Negative regulation of MAPK pathway TAS Reactome
Nervous system development TAS Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Oncogenic MAPK signaling TAS Reactome
p38MAPK events TAS Reactome
p38MAPK events IEA Reactome
Paradoxical activation of RAF signaling by kinase inactive BRAF TAS Reactome
Post NMDA receptor activation events IEA Reactome
PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases TAS Reactome
RAF activation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Ras activation upon Ca2+ influx through NMDA receptor IEA Reactome
RAS GTPase cycle mutants TAS Reactome
RAS processing TAS Reactome
RAS signaling downstream of NF1 loss-of-function variants TAS Reactome
Regulation of RAS by GAPs TAS Reactome
SHC-mediated cascade:FGFR1 TAS Reactome
SHC-mediated cascade:FGFR2 TAS Reactome
SHC-mediated cascade:FGFR3 TAS Reactome
SHC-mediated cascade:FGFR4 TAS Reactome
SHC-related events triggered by IGF1R IEA Reactome
SHC1 events in EGFR signaling TAS Reactome
SHC1 events in ERBB2 signaling IEA Reactome
SHC1 events in ERBB4 signaling TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by BRAF and RAF1 fusions TAS Reactome
Signaling by EGFR TAS Reactome
Signaling by EGFR in Cancer TAS Reactome
Signaling by EGFRvIII in Cancer TAS Reactome
Signaling by ERBB2 IEA Reactome
Signaling by ERBB2 TAS Reactome
Signaling by ERBB2 ECD mutants TAS Reactome
Signaling by ERBB2 in Cancer TAS Reactome
Signaling by ERBB2 KD Mutants TAS Reactome
Signaling by ERBB2 TMD/JMD mutants TAS Reactome
Signaling by ERBB4 TAS Reactome
Signaling by Erythropoietin IEA Reactome
Signaling by FGFR TAS Reactome
Signaling by FGFR in disease TAS Reactome
Signaling by FGFR1 TAS Reactome
Signaling by FGFR1 in disease TAS Reactome
Signaling by FGFR2 TAS Reactome
Signaling by FGFR2 in disease TAS Reactome
Signaling by FGFR3 TAS Reactome
Signaling by FGFR3 in disease TAS Reactome
Signaling by FGFR4 TAS Reactome
Signaling by FGFR4 in disease TAS Reactome
Signaling by FLT3 fusion proteins TAS Reactome
Signaling by FLT3 ITD and TKD mutants TAS Reactome
Signaling by GPCR TAS Reactome
Signaling by high-kinase activity BRAF mutants TAS Reactome
Signaling by Insulin receptor IEA Reactome
Signaling by KIT in disease TAS Reactome
Signaling by Ligand-Responsive EGFR Variants in Cancer TAS Reactome
Signaling by MET TAS Reactome
Signaling by moderate kinase activity BRAF mutants TAS Reactome
Signaling by Non-Receptor Tyrosine Kinases TAS Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRK1 (TRKA) TAS Reactome
Signaling by NTRK2 (TRKB) IEA Reactome
Signaling by NTRK3 (TRKC) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by NTRKs TAS Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by PDGF TAS Reactome
Signaling by PDGFR in disease TAS Reactome
Signaling by PDGFRA extracellular domain mutants TAS Reactome
Signaling by PDGFRA transmembrane, juxtamembrane and kinase domain mutants TAS Reactome
Signaling by phosphorylated juxtamembrane, extracellular and kinase domain KIT mutants TAS Reactome
Signaling by PTK6 TAS Reactome
Signaling by RAF1 mutants TAS Reactome
Signaling by RAS GAP mutants TAS Reactome
Signaling by RAS GTPase mutants TAS Reactome
Signaling by RAS mutants TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by SCF-KIT TAS Reactome
Signaling by the B Cell Receptor (BCR) TAS Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) IEA Reactome
Signaling by VEGF TAS Reactome
Signaling downstream of RAS mutants TAS Reactome
Signalling to ERKs IEA Reactome
Signalling to ERKs TAS Reactome
Signalling to RAS IEA Reactome
Signalling to RAS TAS Reactome
SOS-mediated signalling IEA Reactome
Tie2 Signaling TAS Reactome
Transmission across Chemical Synapses IEA Reactome
VEGFA-VEGFR2 Pathway TAS Reactome
VEGFR2 mediated cell proliferation TAS Reactome





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