Gene description for Rab7
Gene name RAB7, member RAS oncogene family
Gene symbol Rab7
Other names/aliases Rab7a
Species Mus musculus
 Database cross references - Rab7
ExoCarta ExoCarta_19349
Vesiclepedia VP_19349
Entrez Gene 19349
UniProt P51150  
 Rab7 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Dendritic cells 11390481    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Microglia 16081791    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab7
Molecular Function
    GTPase activity GO:0003924 ISO
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 IEA
    GDP binding GO:0019003 ISO
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 ISO
    retromer complex binding GO:1905394 IEA
    retromer complex binding GO:1905394 ISO
Biological Process
    autophagosome assembly GO:0000045 IEA
    protein targeting to lysosome GO:0006622 IEA
    protein targeting to lysosome GO:0006622 ISO
    intracellular protein transport GO:0006886 TAS
    epidermal growth factor catabolic process GO:0007174 IEA
    epidermal growth factor catabolic process GO:0007174 ISO
    endosome to lysosome transport GO:0008333 IBA
    endosome to lysosome transport GO:0008333 ISO
    response to bacterium GO:0009617 IEA
    response to bacterium GO:0009617 ISO
    lipid catabolic process GO:0016042 IEA
    viral release from host cell GO:0019076 IEA
    viral release from host cell GO:0019076 ISO
    protein to membrane docking GO:0022615 IEA
    protein to membrane docking GO:0022615 ISO
    synaptic vesicle recycling via endosome GO:0036466 IEA
    retrograde transport, endosome to Golgi GO:0042147 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    early endosome to late endosome transport GO:0045022 ISO
    early endosome to late endosome transport GO:0045022 ISS
    bone resorption GO:0045453 IEA
    bone resorption GO:0045453 ISO
    positive regulation of protein catabolic process GO:0045732 IEA
    positive regulation of protein catabolic process GO:0045732 ISO
    intracellular transport GO:0046907 ISO
    positive regulation of viral process GO:0048524 IEA
    positive regulation of viral process GO:0048524 ISO
    establishment of vesicle localization GO:0051650 IMP
    lipophagy GO:0061724 IMP
    phagosome acidification GO:0090383 ISO
    phagosome acidification GO:0090383 ISS
    phagosome-lysosome fusion GO:0090385 IBA
    phagosome-lysosome fusion GO:0090385 ISO
    phagosome-lysosome fusion GO:0090385 ISS
    neurotransmitter receptor transport, postsynaptic endosome to lysosome GO:0098943 IDA
    neurotransmitter receptor transport, postsynaptic endosome to lysosome GO:0098943 IMP
    vesicle-mediated transport in synapse GO:0099003 IDA
    vesicle-mediated transport in synapse GO:0099003 IMP
    endosome to plasma membrane protein transport GO:0099638 ISO
    endosome to plasma membrane protein transport GO:0099638 ISS
    negative regulation of exosomal secretion GO:1903542 IEA
    negative regulation of exosomal secretion GO:1903542 ISO
    positive regulation of exosomal secretion GO:1903543 IEA
    positive regulation of exosomal secretion GO:1903543 ISO
Subcellular Localization
    autophagosome membrane GO:0000421 IEA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    lysosome GO:0005764 IDA
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISO
    lysosomal membrane GO:0005765 IDA
    endosome GO:0005768 IDA
    late endosome GO:0005770 IDA
    late endosome GO:0005770 ISO
    late endosome GO:0005770 ISO
    Golgi apparatus GO:0005794 IDA
    lipid droplet GO:0005811 IDA
    cytosol GO:0005829 IDA
    endosome membrane GO:0010008 ISO
    phagocytic vesicle membrane GO:0030670 IEA
    synaptic vesicle membrane GO:0030672 IEA
    synaptic vesicle membrane GO:0030672 ISO
    retromer complex GO:0030904 IEA
    retromer complex GO:0030904 ISO
    late endosome membrane GO:0031902 IDA
    mitochondrial membrane GO:0031966 IEA
    melanosome membrane GO:0033162 IEA
    phagophore assembly site membrane GO:0034045 IDA
    phagocytic vesicle GO:0045335 IBA
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
    alveolar lamellar body GO:0097208 IEA
    alveolar lamellar body GO:0097208 ISO
    presynaptic endosome GO:0098830 IEA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Rab7 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
4
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
6
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPX2  
Affinity Capture-MS Homo sapiens
2 Rab5c 19345
Co-fractionation Mus musculus
3 Lmna 16905
Affinity Capture-MS Mus musculus
4 Snx6 72183
Co-fractionation Mus musculus
5 Vps33a  
Co-fractionation Mus musculus
6 Rab4b  
Co-fractionation Mus musculus
7 Hspb2 69253
Affinity Capture-MS Mus musculus
8 Vps26a 30930
Co-fractionation Mus musculus
9 Rab5b 19344
Co-fractionation Mus musculus
10 Pgrmc1  
Co-fractionation Mus musculus
11 Rabggtb  
Co-fractionation Mus musculus
12 Tsc1  
Affinity Capture-MS Mus musculus
13 SDCCAG8  
Affinity Capture-MS Homo sapiens
14 Kctd13  
Affinity Capture-MS Mus musculus
15 Vps29 56433
Co-fractionation Mus musculus
16 Dnm2 13430
Co-fractionation Mus musculus
17 Snx1  
Co-fractionation Mus musculus
18 Mtss1  
Affinity Capture-Western Mus musculus
19 Tmem173  
Proximity Label-MS Mus musculus
20 SLC38A10 124565
Affinity Capture-MS Homo sapiens
21 Rpa1 68275
Co-fractionation Mus musculus
22 Rheb  
Co-fractionation Mus musculus
23 PDS5A 23244
Affinity Capture-MS Homo sapiens
24 Sh3kbp1 58194
Co-fractionation Mus musculus
25 Vps35 65114
Co-fractionation Mus musculus
26 Uchl5 56207
Co-fractionation Mus musculus
27 Snx2 67804
Co-fractionation Mus musculus
28 Sstr3  
Proximity Label-MS Mus musculus
29 AAMDC  
Affinity Capture-MS Homo sapiens
30 Eed  
Affinity Capture-MS Mus musculus
31 CHM 1121
Affinity Capture-MS Homo sapiens
32 Rnf115  
PCA Mus musculus
33 Atg12  
Negative Genetic Mus musculus
34 Rab5a 271457
Co-fractionation Mus musculus
35 Fancd2  
Affinity Capture-MS Mus musculus
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