Gene description for Dnm2
Gene name dynamin 2
Gene symbol Dnm2
Other names/aliases Dyn2
Udnm
b2b2159Clo
Species Mus musculus
 Database cross references - Dnm2
ExoCarta ExoCarta_13430
Vesiclepedia VP_13430
Entrez Gene 13430
UniProt P39054  
 Dnm2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dnm2
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISO
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISS
    microtubule binding GO:0008017 IBA
    SH3 domain binding GO:0017124 ISO
    protein kinase binding GO:0019901 ISO
    D2 dopamine receptor binding GO:0031749 ISO
    phosphatidylinositol 3-kinase regulatory subunit binding GO:0036312 ISO
    protein-containing complex binding GO:0044877 ISO
    WW domain binding GO:0050699 ISO
    nitric-oxide synthase binding GO:0050998 ISO
    protein serine/threonine kinase binding GO:0120283 ISO
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    G protein-coupled receptor internalization GO:0002031 ISO
    ventricular septum development GO:0003281 IMP
    Golgi to plasma membrane transport GO:0006893 ISO
    endocytosis GO:0006897 ISO
    receptor-mediated endocytosis GO:0006898 ISO
    receptor-mediated endocytosis GO:0006898 ISS
    phagocytosis GO:0006909 IEA
    autophagy GO:0006914 ISO
    autophagy GO:0006914 ISS
    centrosome cycle GO:0007098 ISO
    centrosome cycle GO:0007098 ISS
    positive regulation of lamellipodium assembly GO:0010592 ISO
    synaptic vesicle budding from presynaptic endocytic zone membrane GO:0016185 IBA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 ISO
    regulation of axon extension GO:0030516 ISO
    regulation of axon extension GO:0030516 ISS
    receptor internalization GO:0031623 IBA
    receptor internalization GO:0031623 ISO
    transferrin transport GO:0033572 ISO
    regulation of Rac protein signal transduction GO:0035020 ISO
    aorta development GO:0035904 IMP
    stress fiber assembly GO:0043149 ISO
    stress fiber assembly GO:0043149 ISS
    macropinocytosis GO:0044351 ISO
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISO
    positive regulation of endocytosis GO:0045807 ISO
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    neuron projection morphogenesis GO:0048812 ISO
    neuron projection morphogenesis GO:0048812 ISS
    positive regulation of phagocytosis GO:0050766 ISO
    protein polymerization GO:0051258 ISO
    protein polymerization GO:0051258 ISS
    coronary vasculature development GO:0060976 IMP
    actin filament bundle organization GO:0061572 ISO
    actin filament bundle organization GO:0061572 ISS
    membrane tubulation GO:0097749 ISO
    membrane tubulation GO:0097749 ISS
    vesicle scission GO:0099050 ISO
    vesicle scission GO:0099050 ISS
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    negative regulation of non-motile cilium assembly GO:1902856 ISO
    cellular response to dopamine GO:1903351 ISO
    regulation of Golgi organization GO:1903358 ISO
    negative regulation of membrane tubulation GO:1903526 ISO
    positive regulation of clathrin-dependent endocytosis GO:2000370 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    phagocytic cup GO:0001891 IEA
    phagocytic cup GO:0001891 ISO
    photoreceptor inner segment GO:0001917 IDA
    uropod GO:0001931 ISO
    uropod GO:0001931 ISS
    podosome GO:0002102 IEA
    cytoplasm GO:0005737 IBA
    endosome GO:0005768 ISO
    endosome GO:0005768 ISS
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 ISO
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    centriole GO:0005814 ISO
    centriole GO:0005814 ISS
    cytosol GO:0005829 ISO
    microtubule GO:0005874 IBA
    microtubule GO:0005874 ISO
    microtubule GO:0005874 ISS
    plasma membrane GO:0005886 IBA
    clathrin-coated pit GO:0005905 IDA
    clathrin-coated pit GO:0005905 ISO
    clathrin-coated pit GO:0005905 ISS
    postsynaptic density GO:0014069 ISO
    postsynaptic density GO:0014069 ISS
    lamellipodium GO:0030027 ISO
    cell junction GO:0030054 ISO
    cell junction GO:0030054 ISS
    clathrin-coated vesicle GO:0030136 ISO
    clathrin-coated vesicle GO:0030136 ISS
    growth cone GO:0030426 ISO
    growth cone GO:0030426 ISS
    midbody GO:0030496 IEA
    phagocytic vesicle membrane GO:0030670 IEA
    ruffle membrane GO:0032587 ISO
    protein-containing complex GO:0032991 ISO
    neuron projection GO:0043005 ISO
    neuron projection GO:0043005 ISS
    synapse GO:0045202 IBA
    postsynaptic membrane GO:0045211 ISO
    clathrin-coated endocytic vesicle GO:0045334 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    recycling endosome GO:0055037 ISO
    recycling endosome GO:0055037 ISS
    anchoring junction GO:0070161 IEA
    presynapse GO:0098793 IEA
    glutamatergic synapse GO:0098978 ISO
    postsynaptic density, intracellular component GO:0099092 ISO
 Experiment description of studies that identified Dnm2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dnm2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
2 Pacsin1  
Affinity Capture-Western Mus musculus
3 Stau1  
Co-fractionation Mus musculus
4 Amph  
Reconstituted Complex Mus musculus
5 Foxp3  
Affinity Capture-MS Mus musculus
6 Kctd13  
Affinity Capture-MS Mus musculus
7 Asap1  
Co-fractionation Mus musculus
8 Rab7 19349
Co-fractionation Mus musculus
9 Pacsin3 80708
Affinity Capture-Western Mus musculus
10 Gab1  
Co-fractionation Mus musculus
11 Cyfip1 20430
Affinity Capture-MS Mus musculus
12 Atg5  
Affinity Capture-MS Mus musculus
13 MYOF 26509
Reconstituted Complex Homo sapiens
14 Sh3kbp1 58194
Co-fractionation Mus musculus
15 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
16 Eed  
Affinity Capture-MS Mus musculus
17 Pacsin2 23970
Affinity Capture-Western Mus musculus
18 Zfand6 65098
Co-fractionation Mus musculus
19 Set 56086
Affinity Capture-MS Mus musculus
20 Wipi2 74781
Co-fractionation Mus musculus
21 Fancd2  
Affinity Capture-MS Mus musculus
22 Bre  
Co-fractionation Mus musculus
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