Gene description for CTSL
Gene name cathepsin L
Gene symbol CTSL
Other names/aliases CATL
CTSL1
MEP
Species Homo sapiens
 Database cross references - CTSL
ExoCarta ExoCarta_1514
Vesiclepedia VP_1514
Entrez Gene 1514
HGNC 2537
MIM 116880
UniProt P07711  
 CTSL identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Keratinocytes 19530224    
Keratinocytes 19530224    
 Gene ontology annotations for CTSL
Molecular Function
    fibronectin binding GO:0001968 IPI
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type endopeptidase activity GO:0004197 IDA
    cysteine-type endopeptidase activity GO:0004197 IMP
    cysteine-type endopeptidase activity GO:0004197 TAS
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IDA
    cysteine-type peptidase activity GO:0008234 IDA
    histone binding GO:0042393 IDA
    proteoglycan binding GO:0043394 IPI
    serpin family protein binding GO:0097655 IPI
Biological Process
    adaptive immune response GO:0002250 IEP
    proteolysis GO:0006508 IDA
    protein autoprocessing GO:0016540 IDA
    fusion of virus membrane with host plasma membrane GO:0019064 IDA
    receptor-mediated endocytosis of virus by host cell GO:0019065 IDA
    antigen processing and presentation GO:0019882 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    collagen catabolic process GO:0030574 IDA
    zymogen activation GO:0031638 IDA
    enkephalin processing GO:0034230 ISS
    fusion of virus membrane with host endosome membrane GO:0039654 IDA
    CD4-positive, alpha-beta T cell lineage commitment GO:0043373 ISS
    symbiont entry into host cell GO:0046718 IDA
    antigen processing and presentation of peptide antigen GO:0048002 ISS
    proteolysis involved in protein catabolic process GO:0051603 IBA
    proteolysis involved in protein catabolic process GO:0051603 IDA
    elastin catabolic process GO:0060309 ISS
    macrophage apoptotic process GO:0071888 NAS
    cellular response to thyroid hormone stimulus GO:0097067 IEP
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 TAS
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    multivesicular body GO:0005771 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IDA
    apical plasma membrane GO:0016324 IEA
    endolysosome lumen GO:0036021 TAS
    chromaffin granule GO:0042583 ISS
    lysosomal lumen GO:0043202 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    endocytic vesicle lumen GO:0071682 TAS
 Experiment description of studies that identified CTSL in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CTSL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CST1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SERPINB3 6317
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
3 CTSK 1513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CLTCL1 8218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CTSV 1515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 HPN  
Affinity Capture-MS Homo sapiens
7 ALB 213
Affinity Capture-MS Homo sapiens
8 MEX3A  
Affinity Capture-RNA Homo sapiens
9 CST3 1471
Affinity Capture-MS Homo sapiens
10 CTSL3P  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CSTA 1475
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
12 CST6  
Affinity Capture-MS Homo sapiens
13 BTN3A3 10384
Two-hybrid Homo sapiens
14 NUP35 129401
Proximity Label-MS Homo sapiens
15 DDX58 23586
Affinity Capture-RNA Homo sapiens
16 CST7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 NXF1 10482
Affinity Capture-RNA Homo sapiens
18 BAG6 7917
Two-hybrid Homo sapiens
19 PPP1CC 5501
Two-hybrid Homo sapiens
20 GEM  
Two-hybrid Homo sapiens
21 MOV10 4343
Affinity Capture-RNA Homo sapiens
22 PGD 5226
Co-fractionation Homo sapiens
23 RXRA 6256
Biochemical Activity Homo sapiens
24 RELB  
Two-hybrid Homo sapiens
25 IGF2R 3482
Affinity Capture-MS Homo sapiens
26 KNG1 3827
Affinity Capture-MS Homo sapiens
27 SGTA 6449
Two-hybrid Homo sapiens
28 CCDC88A 55704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 EED  
Two-hybrid Homo sapiens
30 CSTB 1476
Affinity Capture-MS Homo sapiens
31 TMPRSS13  
Affinity Capture-MS Homo sapiens
32 APOA1 335
Affinity Capture-MS Homo sapiens
33 CTSH 1512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here