Gene description for Dbt
Gene name dihydrolipoamide branched chain transacylase E2
Gene symbol Dbt
Other names/aliases D3Wsu60e
Species Mus musculus
 Database cross references - Dbt
ExoCarta ExoCarta_13171
Vesiclepedia VP_13171
Entrez Gene 13171
UniProt P53395  
 Dbt identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Dbt
Molecular Function
    acetyltransferase activity GO:0016407 IBA
    lipoic acid binding GO:0031405 IBA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity GO:0043754 IEA
    branched-chain alpha-keto acid dehydrogenase activity GO:0047101 IEA
    branched-chain alpha-keto acid dehydrogenase activity GO:0047101 ISO
Biological Process
    branched-chain amino acid catabolic process GO:0009083 IEA
    branched-chain amino acid catabolic process GO:0009083 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    microtubule cytoskeleton GO:0015630 IEA
    microtubule cytoskeleton GO:0015630 ISO
    mitochondrial nucleoid GO:0042645 IEA
    mitochondrial nucleoid GO:0042645 ISO
    branched-chain alpha-ketoacid dehydrogenase complex GO:0160157 IEA
 Experiment description of studies that identified Dbt in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Dbt
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cebpa  
Affinity Capture-MS Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
4 Foxp3  
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Atg16l1  
Affinity Capture-MS Mus musculus
7 HSD17B10 3028
Affinity Capture-MS Homo sapiens
8 Pou5f1  
Affinity Capture-MS Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
10 Dbt 13171
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
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