Gene description for Cstb
Gene name cystatin B
Gene symbol Cstb
Other names/aliases AA960480
Stfb
Species Mus musculus
 Database cross references - Cstb
ExoCarta ExoCarta_13014
Vesiclepedia VP_13014
Entrez Gene 13014
UniProt Q62426  
 Cstb identified in sEVs derived from the following tissue/cell type
Microglia 16081791    
 Gene ontology annotations for Cstb
Molecular Function
    protease binding GO:0002020 ISO
    endopeptidase inhibitor activity GO:0004866 ISO
    cysteine-type endopeptidase inhibitor activity GO:0004869 IBA
    cysteine-type endopeptidase inhibitor activity GO:0004869 IEA
    cysteine-type endopeptidase inhibitor activity GO:0004869 ISO
Biological Process
    adult locomotory behavior GO:0008344 IMP
    negative regulation of peptidase activity GO:0010466 ISO
    negative regulation of proteolysis GO:0045861 IEA
    negative regulation of proteolysis GO:0045861 ISO
    amyloid fibril formation GO:1990000 IEA
Subcellular Localization
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Cstb in sEVs
1
Experiment ID 14
MISEV standards
EM
Biophysical techniques
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
DNM
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Cstb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Anxa4 11746
Co-fractionation Mus musculus
2 Slc4a2  
Co-fractionation Mus musculus
3 B2m 12010
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Cstb is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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