Gene description for Krt5
Gene name keratin 5
Gene symbol Krt5
Other names/aliases 3300001P10Rik
AW146334
CK5
K5
Krt2-5
Tfip8
Species Mus musculus
 Database cross references - Krt5
ExoCarta ExoCarta_110308
Vesiclepedia VP_110308
Entrez Gene 110308
UniProt Q922U2  
 Krt5 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
 Gene ontology annotations for Krt5
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of skin epidermis GO:0030280 IBA
    scaffold protein binding GO:0097110 ISO
Biological Process
    response to mechanical stimulus GO:0009612 IMP
    regulation of cell migration GO:0030334 IMP
    keratinization GO:0031424 IBA
    regulation of protein localization GO:0032880 IMP
    intermediate filament polymerization GO:0045107 IMP
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cornified envelope GO:0001533 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    intermediate filament GO:0005882 ISO
    plasma membrane GO:0005886 IDA
    keratin filament GO:0045095 IBA
    keratin filament GO:0045095 IDA
    keratin filament GO:0045095 ISO
 Experiment description of studies that identified Krt5 in sEVs
1
Experiment ID 15
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 33
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 188
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Krt5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nphp3  
Affinity Capture-MS Mus musculus
2 Invs  
Affinity Capture-MS Mus musculus
3 Eps15 13858
Co-fractionation Mus musculus
4 Nphp4  
Affinity Capture-MS Mus musculus
5 Inppl1  
Co-fractionation Mus musculus
6 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Thrb  
Affinity Capture-MS Mus musculus
9 Cep290  
Affinity Capture-MS Mus musculus
10 Ahi1  
Affinity Capture-MS Mus musculus
11 Ubash3b  
Co-fractionation Mus musculus
12 Nphp1  
Affinity Capture-MS Mus musculus
13 Mks1  
Affinity Capture-MS Mus musculus
14 Iqcb1  
Affinity Capture-MS Mus musculus
15 Nanog  
Affinity Capture-MS Mus musculus
16 Krt17 16667
Co-fractionation Mus musculus
17 Evpl  
Co-fractionation Mus musculus
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