Gene description for FRS2
Gene name fibroblast growth factor receptor substrate 2
Gene symbol FRS2
Other names/aliases FRS2A
FRS2alpha
SNT
SNT-1
SNT1
Species Homo sapiens
 Database cross references - FRS2
ExoCarta ExoCarta_10818
Vesiclepedia VP_10818
Entrez Gene 10818
HGNC 16971
MIM 607743
UniProt Q8WU20  
 FRS2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for FRS2
Molecular Function
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 IBA
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 TAS
    fibroblast growth factor receptor binding GO:0005104 IBA
    fibroblast growth factor receptor binding GO:0005104 IPI
    neurotrophin TRKA receptor binding GO:0005168 IPI
    protein binding GO:0005515 IPI
    phosphatase activator activity GO:0019211 TAS
Biological Process
    gastrulation with mouth forming second GO:0001702 IEA
    organ induction GO:0001759 IEA
    ventricular septum development GO:0003281 IEA
    cell surface receptor protein tyrosine phosphatase signaling pathway GO:0007185 TAS
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    neuroblast proliferation GO:0007405 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IBA
    fibroblast growth factor receptor signaling pathway GO:0008543 IGI
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    anterior/posterior axis specification, embryo GO:0008595 IEA
    forebrain development GO:0030900 IEA
    regulation of apoptotic process GO:0042981 IEA
    positive regulation of MAPK cascade GO:0043410 IEA
    lens placode formation involved in camera-type eye formation GO:0046619 IEA
    regulation of epithelial cell proliferation GO:0050678 IEA
    prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis GO:0060527 IEA
    lens fiber cell development GO:0070307 IEA
    regulation of ERK1 and ERK2 cascade GO:0070372 IEA
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IEA
    negative regulation of cardiac muscle cell differentiation GO:2000726 IGI
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IBA
    adherens junction GO:0005912 IDA
    endomembrane system GO:0012505 IEA
    membrane GO:0016020 TAS
 Experiment description of studies that identified FRS2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for FRS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANLN 54443
Affinity Capture-MS Homo sapiens
2 RNF43  
Proximity Label-MS Homo sapiens
3 CCDC94  
Two-hybrid Homo sapiens
4 GJD3  
Proximity Label-MS Homo sapiens
5 RAB35 11021
Proximity Label-MS Homo sapiens
6 PTPN11 5781
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
7 Grb2 14784
Affinity Capture-Western Mus musculus
8 FGFR1 2260
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
9 MCAM 4162
Proximity Label-MS Homo sapiens
10 GRB2 2885
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 MARCKS 4082
Proximity Label-MS Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 SORBS1 10580
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 RAB9A 9367
Proximity Label-MS Homo sapiens
15 RAB5A 5868
Proximity Label-MS Homo sapiens
16 GJA1 2697
Proximity Label-MS Homo sapiens
17 NTRK2 4915
Reconstituted Complex Homo sapiens
18 RB1 5925
Negative Genetic Homo sapiens
19 PIN1 5300
Two-hybrid Homo sapiens
20 PARD3 56288
Proximity Label-MS Homo sapiens
21 CRK 1398
Reconstituted Complex Homo sapiens
22 EZR 7430
Proximity Label-MS Homo sapiens
23 LCK 3932
Proximity Label-MS Homo sapiens
24 CDK3 1018
Two-hybrid Homo sapiens
25 SOS1 6654
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 INSR 3643
Biochemical Activity Homo sapiens
27 Gab1  
Affinity Capture-Western Mus musculus
28 ARF6 382
Proximity Label-MS Homo sapiens
29 RET 5979
Reconstituted Complex Homo sapiens
30 PDGFRB 5159
Affinity Capture-Western Homo sapiens
31 CXADR 1525
Proximity Label-MS Homo sapiens
32 STX4 6810
Proximity Label-MS Homo sapiens
33 C11orf52 91894
Proximity Label-MS Homo sapiens
34 OCLN 100506658
Proximity Label-MS Homo sapiens
35 LYN 4067
Proximity Label-MS Homo sapiens
36 GAB1  
Affinity Capture-Western Homo sapiens
37 DNAJC5 80331
Proximity Label-MS Homo sapiens
38 CBL 867
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 RAB4A 5867
Proximity Label-MS Homo sapiens
40 PRKCI 5584
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
41 LAMTOR1 55004
Proximity Label-MS Homo sapiens
42 FGFR2 2263
Affinity Capture-Western Homo sapiens
43 NTRK3 4916
Reconstituted Complex Homo sapiens
44 CDH1 999
Proximity Label-MS Homo sapiens
45 MLLT4 4301
Proximity Label-MS Homo sapiens
46 DIRAS3  
Proximity Label-MS Homo sapiens
47 NTRK1 4914
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
48 EPB41L4A 64097
Proximity Label-MS Homo sapiens
49 RAB11A 8766
Proximity Label-MS Homo sapiens
50 EPHA2 1969
Proximity Label-MS Homo sapiens
51 EBAG9 9166
Proximity Label-MS Homo sapiens
52 RHOB 388
Proximity Label-MS Homo sapiens
53 Coro1c 23790
Affinity Capture-MS Mus musculus
54 GAB2 9846
Affinity Capture-Western Homo sapiens
55 SPRY2 10253
Affinity Capture-Western Homo sapiens
56 CAV1 857
Proximity Label-MS Homo sapiens
57 KRAS 3845
Proximity Label-MS Homo sapiens
58 FLOT1 10211
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
59 STX6 10228
Proximity Label-MS Homo sapiens
60 Ptpn11 19247
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which FRS2 is involved
PathwayEvidenceSource
Activated NTRK2 signals through FRS2 and FRS3 IEA Reactome
Activated NTRK2 signals through FRS2 and FRS3 TAS Reactome
Axon guidance TAS Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Downstream signaling of activated FGFR1 TAS Reactome
Downstream signaling of activated FGFR2 TAS Reactome
Downstream signaling of activated FGFR3 TAS Reactome
Downstream signaling of activated FGFR4 TAS Reactome
FRS-mediated FGFR1 signaling TAS Reactome
FRS-mediated FGFR2 signaling TAS Reactome
FRS-mediated FGFR3 signaling TAS Reactome
FRS-mediated FGFR4 signaling TAS Reactome
Frs2-mediated activation IEA Reactome
Frs2-mediated activation TAS Reactome
IGF1R signaling cascade TAS Reactome
Insulin receptor signalling cascade TAS Reactome
Intracellular signaling by second messengers TAS Reactome
IRS-mediated signalling TAS Reactome
IRS-related events triggered by IGF1R TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Negative regulation of FGFR1 signaling TAS Reactome
Negative regulation of FGFR2 signaling TAS Reactome
Negative regulation of FGFR3 signaling TAS Reactome
Negative regulation of FGFR4 signaling TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
Nervous system development TAS Reactome
PI-3K cascade:FGFR1 TAS Reactome
PI-3K cascade:FGFR2 TAS Reactome
PI-3K cascade:FGFR3 TAS Reactome
PI-3K cascade:FGFR4 TAS Reactome
PI3K Cascade TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Prolonged ERK activation events IEA Reactome
Prolonged ERK activation events TAS Reactome
RAF/MAP kinase cascade TAS Reactome
RET signaling TAS Reactome
RHO GTPase cycle TAS Reactome
RND1 GTPase cycle TAS Reactome
RND2 GTPase cycle TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by ALK IEA Reactome
Signaling by ALK fusions and activated point mutants TAS Reactome
Signaling by ALK in cancer TAS Reactome
Signaling by FGFR TAS Reactome
Signaling by FGFR in disease TAS Reactome
Signaling by FGFR1 TAS Reactome
Signaling by FGFR1 in disease TAS Reactome
Signaling by FGFR2 TAS Reactome
Signaling by FGFR2 in disease TAS Reactome
Signaling by FGFR3 TAS Reactome
Signaling by FGFR3 in disease TAS Reactome
Signaling by FGFR4 TAS Reactome
Signaling by FGFR4 in disease TAS Reactome
Signaling by Insulin receptor TAS Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRK1 (TRKA) TAS Reactome
Signaling by NTRK2 (TRKB) IEA Reactome
Signaling by NTRK2 (TRKB) TAS Reactome
Signaling by NTRKs IEA Reactome
Signaling by NTRKs TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) TAS Reactome
Signalling to ERKs IEA Reactome
Signalling to ERKs TAS Reactome





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