Gene description for Shmt2
Gene name serine hydroxymethyltransferase 2 (mitochondrial)
Gene symbol Shmt2
Other names/aliases 2700043D08Rik
AA408223
AA986903
Species Mus musculus
 Database cross references - Shmt2
ExoCarta ExoCarta_108037
Vesiclepedia VP_108037
Entrez Gene 108037
 Shmt2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Shmt2
Molecular Function
    chromatin binding GO:0003682 IEA
    chromatin binding GO:0003682 ISO
    glycine hydroxymethyltransferase activity GO:0004372 IBA
    glycine hydroxymethyltransferase activity GO:0004372 IGI
    glycine hydroxymethyltransferase activity GO:0004372 IMP
    glycine hydroxymethyltransferase activity GO:0004372 ISO
    glycine hydroxymethyltransferase activity GO:0004372 ISS
    L-allo-threonine aldolase activity GO:0008732 IEA
    L-allo-threonine aldolase activity GO:0008732 ISO
    amino acid binding GO:0016597 IEA
    amino acid binding GO:0016597 ISO
    pyridoxal phosphate binding GO:0030170 IBA
    pyridoxal phosphate binding GO:0030170 IEA
    pyridoxal phosphate binding GO:0030170 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    regulation of oxidative phosphorylation GO:0002082 IMP
    regulation of oxidative phosphorylation GO:0002082 ISO
    glycine metabolic process GO:0006544 ISO
    glycine metabolic process GO:0006544 ISS
    glycine biosynthetic process GO:0006545 ISO
    L-serine metabolic process GO:0006563 ISO
    L-serine metabolic process GO:0006563 ISS
    L-serine biosynthetic process GO:0006564 IEA
    L-serine biosynthetic process GO:0006564 ISO
    one-carbon metabolic process GO:0006730 ISO
    one-carbon metabolic process GO:0006730 ISS
    positive regulation of cell population proliferation GO:0008284 IEA
    positive regulation of cell population proliferation GO:0008284 ISO
    glycine biosynthetic process from serine GO:0019264 IBA
    glycine biosynthetic process from serine GO:0019264 IEA
    glycine biosynthetic process from serine GO:0019264 ISO
    response to type I interferon GO:0034340 IEA
    response to type I interferon GO:0034340 ISO
    tetrahydrofolate interconversion GO:0035999 IEA
    tetrahydrofolate interconversion GO:0035999 IMP
    tetrahydrofolate metabolic process GO:0046653 IBA
    tetrahydrofolate metabolic process GO:0046653 ISO
    protein tetramerization GO:0051262 ISO
    protein homotetramerization GO:0051289 ISO
    protein homotetramerization GO:0051289 ISS
    regulation of mitochondrial translation GO:0070129 IMP
    regulation of mitochondrial translation GO:0070129 ISO
    protein K63-linked deubiquitination GO:0070536 IEA
    protein K63-linked deubiquitination GO:0070536 ISO
    regulation of aerobic respiration GO:1903715 IMP
    regulation of aerobic respiration GO:1903715 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IGI
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial matrix GO:0005759 ISO
    microtubule cytoskeleton GO:0015630 IEA
    microtubule cytoskeleton GO:0015630 ISO
    mitochondrial nucleoid GO:0042645 IEA
    mitochondrial nucleoid GO:0042645 ISO
    BRISC complex GO:0070552 IEA
    BRISC complex GO:0070552 ISO
 Experiment description of studies that identified Shmt2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Shmt2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Set 56086
Affinity Capture-MS Mus musculus
2 Shmt2 108037
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml



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