Gene description for Ndufv2
Gene name NADH dehydrogenase (ubiquinone) flavoprotein 2
Gene symbol Ndufv2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ndufv2
ExoCarta ExoCarta_81728
Vesiclepedia VP_81728
Entrez Gene 81728
UniProt P19234  
 Ndufv2 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Ndufv2
Molecular Function
    NADH dehydrogenase activity GO:0003954 IBA
    NADH dehydrogenase (ubiquinone) activity GO:0008137 IEA
    NADH dehydrogenase (ubiquinone) activity GO:0008137 ISO
    NADH dehydrogenase (ubiquinone) activity GO:0008137 ISO
    NADH dehydrogenase (ubiquinone) activity GO:0008137 ISS
    metal ion binding GO:0046872 IEA
    2 iron, 2 sulfur cluster binding GO:0051537 IEA
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 IBA
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 IEA
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 ISO
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 ISS
    nervous system development GO:0007399 ISO
    biological_process GO:0008150 ND
    cardiac muscle tissue development GO:0048738 ISO
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial inner membrane GO:0005743 ISS
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IEA
    respiratory chain complex I GO:0045271 ISO
    respiratory chain complex I GO:0045271 ISS
 Experiment description of studies that identified Ndufv2 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ndufv2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 STXBP5L  
Affinity Capture-MS Homo sapiens
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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