Gene description for Arhgdia
Gene name Rho GDP dissociation inhibitor (GDI) alpha
Gene symbol Arhgdia
Other names/aliases Bles01
Species Rattus norvegicus
 Database cross references - Arhgdia
ExoCarta ExoCarta_360678
Vesiclepedia VP_360678
Entrez Gene 360678
UniProt Q5XI73  
 Arhgdia identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Arhgdia
Molecular Function
    Rho GDP-dissociation inhibitor activity GO:0005094 IBA
    Rho GDP-dissociation inhibitor activity GO:0005094 IEA
    Rho GDP-dissociation inhibitor activity GO:0005094 ISO
    GTPase activator activity GO:0005096 IEA
    fatty acid binding GO:0005504 IDA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IDA
    small GTPase binding GO:0031267 IPI
    Hsp90 protein binding GO:0051879 IDA
Biological Process
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 IEA
    Rho protein signal transduction GO:0007266 ISO
    regulation of cell shape GO:0008360 IMP
    negative regulation of cell migration GO:0030336 IMP
    regulation of protein localization GO:0032880 ISO
    regulation of Rho protein signal transduction GO:0035023 ISO
    cellular response to mechanical stimulus GO:0071260 IMP
    cellular response to organic cyclic compound GO:0071407 IEP
    cellular response to redox state GO:0071461 IEP
    semaphorin-plexin signaling pathway GO:0071526 ISO
    semaphorin-plexin signaling pathway GO:0071526 ISS
    regulation of synaptic vesicle cycle GO:0098693 ISO
Subcellular Localization
    immunological synapse GO:0001772 ISO
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 IBA
    membrane GO:0016020 IBA
    membrane GO:0016020 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    Schaffer collateral - CA1 synapse GO:0098685 ISO
 Experiment description of studies that identified Arhgdia in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Arhgdia
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sumo3  
Affinity Capture-MS Rattus norvegicus
2 Park7 117287
Affinity Capture-MS Rattus norvegicus
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