Gene description for Acta1
Gene name actin, alpha 1, skeletal muscle
Gene symbol Acta1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Acta1
ExoCarta ExoCarta_29437
Vesiclepedia VP_29437
Entrez Gene 29437
UniProt P68136  
 Acta1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Acta1
Molecular Function
    ATP binding GO:0005524 IEA
    hydrolase activity GO:0016787 IEA
    protein kinase binding GO:0019901 IEA
Biological Process
    regulation of blood pressure GO:0008217 IEA
    response to mechanical stimulus GO:0009612 IEP
    response to virus GO:0009615 IEA
    positive regulation of gene expression GO:0010628 ISS
    vascular associated smooth muscle contraction GO:0014829 IEA
    skeletal muscle thin filament assembly GO:0030240 IBA
    skeletal muscle thin filament assembly GO:0030240 ISO
    skeletal muscle thin filament assembly GO:0030240 ISS
    cellular response to potassium ion GO:0035865 IEP
    skeletal muscle fiber adaptation GO:0043503 IEP
    response to steroid hormone GO:0048545 IEP
    skeletal muscle fiber development GO:0048741 ISO
    skeletal muscle fiber development GO:0048741 ISS
    glomerular mesangial cell development GO:0072144 IEA
    mesenchyme migration GO:0090131 ISS
Subcellular Localization
    stress fiber GO:0001725 IBA
    stress fiber GO:0001725 ISO
    stress fiber GO:0001725 ISS
    cytoplasm GO:0005737 ISS
    striated muscle thin filament GO:0005865 IBA
    striated muscle thin filament GO:0005865 ISO
    striated muscle thin filament GO:0005865 ISS
    actin filament GO:0005884 IBA
    actin filament GO:0005884 ISO
    actin filament GO:0005884 ISS
    actin cytoskeleton GO:0015629 IBA
    actin cytoskeleton GO:0015629 IEA
    actin cytoskeleton GO:0015629 ISO
    sarcomere GO:0030017 ISO
    lamellipodium GO:0030027 ISS
    filopodium GO:0030175 ISS
    smooth muscle contractile fiber GO:0030485 IEA
    motile cilium GO:0031514 IEA
    protein-containing complex GO:0032991 IEA
    cell body GO:0044297 ISS
 Experiment description of studies that identified Acta1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Acta1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snca  
Affinity Capture-MS Rattus norvegicus
2 Sumo3  
Affinity Capture-MS Rattus norvegicus
3 Gja1  
Affinity Capture-MS Rattus norvegicus
4 Park7 117287
Affinity Capture-MS Rattus norvegicus
5 Cnn1  
FRET Rattus norvegicus
View the network image/svg+xml
 Pathways in which Acta1 is involved
PathwayEvidenceSource
Muscle contraction IEA Reactome
Striated Muscle Contraction IEA Reactome





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