Gene description for Slc2a1
Gene name solute carrier family 2 (facilitated glucose transporter), member 1
Gene symbol Slc2a1
Other names/aliases GLUTB
GTG1
Glut1
Gtg3
RATGTG1
Species Rattus norvegicus
 Database cross references - Slc2a1
ExoCarta ExoCarta_24778
Vesiclepedia VP_24778
Entrez Gene 24778
UniProt P11167  
 Slc2a1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Slc2a1
Molecular Function
    long-chain fatty acid transmembrane transporter activity GO:0005324 ISO
    protein binding GO:0005515 IPI
    fucose transmembrane transporter activity GO:0015150 ISO
    kinase binding GO:0019900 IPI
    dehydroascorbic acid transmembrane transporter activity GO:0033300 IDA
    dehydroascorbic acid transmembrane transporter activity GO:0033300 ISO
    identical protein binding GO:0042802 ISO
    xenobiotic transmembrane transporter activity GO:0042910 IDA
    D-glucose transmembrane transporter activity GO:0055056 IBA
    D-glucose transmembrane transporter activity GO:0055056 IDA
    D-glucose transmembrane transporter activity GO:0055056 IEA
    D-glucose transmembrane transporter activity GO:0055056 ISO
Biological Process
    response to hypoxia GO:0001666 IEP
    central nervous system development GO:0007417 ISO
    female pregnancy GO:0007565 IEP
    long-chain fatty acid import across plasma membrane GO:0015911 ISO
    cerebral cortex development GO:0021987 IEP
    response to insulin GO:0032868 IBA
    response to insulin GO:0032868 IEP
    cellular response to glucose starvation GO:0042149 ISO
    xenobiotic transport GO:0042908 IDA
    photoreceptor cell maintenance GO:0045494 ISO
    photoreceptor cell maintenance GO:0045494 ISS
    D-glucose import GO:0046323 IBA
    D-glucose import GO:0046323 IMP
    protein-containing complex assembly GO:0065003 ISO
    protein-containing complex assembly GO:0065003 ISS
    dehydroascorbic acid transport GO:0070837 IBA
    dehydroascorbic acid transport GO:0070837 IDA
    dehydroascorbic acid transport GO:0070837 ISO
    cellular response to mechanical stimulus GO:0071260 IEP
    cellular hyperosmotic response GO:0071474 IEP
    D-glucose import across plasma membrane GO:0098708 ISO
    transport across blood-brain barrier GO:0150104 ISO
    transport across blood-brain barrier GO:0150104 ISO
    response to Thyroglobulin triiodothyronine GO:1904016 IEP
    D-glucose transmembrane transport GO:1904659 IDA
    D-glucose transmembrane transport GO:1904659 IEA
    D-glucose transmembrane transport GO:1904659 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    female germ cell nucleus GO:0001674 ISO
    photoreceptor inner segment GO:0001917 IEA
    photoreceptor inner segment GO:0001917 ISO
    female pronucleus GO:0001939 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    caveola GO:0005901 IDA
    cell-cell junction GO:0005911 IDA
    intercalated disc GO:0014704 IDA
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    Z disc GO:0030018 IDA
    midbody GO:0030496 ISO
    cortical actin cytoskeleton GO:0030864 ISO
    cortical actin cytoskeleton GO:0030864 ISS
    vesicle GO:0031982 ISO
    sarcolemma GO:0042383 IDA
    membrane raft GO:0045121 ISO
    synapse GO:0045202 ISO
    presynapse GO:0098793 ISO
    glucose transporter complex GO:1990350 ISO
 Experiment description of studies that identified Slc2a1 in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Slc2a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
2 Ubc  
Affinity Capture-Western Rattus norvegicus
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