Gene description for Sept2
Gene name septin 2
Gene symbol Sept2
Other names/aliases Nedd5
Vesp11
Species Rattus norvegicus
 Database cross references - Sept2
ExoCarta ExoCarta_117515
Vesiclepedia VP_117515
Entrez Gene 117515
UniProt Q91Y81  
 Sept2 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Sept2
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 TAS
    GTP binding GO:0005525 IEA
    enzyme regulator activity GO:0030234 IEA
    enzyme regulator activity GO:0030234 ISO
    identical protein binding GO:0042802 ISO
    molecular adaptor activity GO:0060090 IBA
Biological Process
    regulation of L-glutamate import across plasma membrane GO:0002036 IEA
    regulation of L-glutamate import across plasma membrane GO:0002036 ISO
    smoothened signaling pathway GO:0007224 IEA
    smoothened signaling pathway GO:0007224 ISO
    smoothened signaling pathway GO:0007224 ISS
    spermatogenesis GO:0007283 IEA
    protein localization GO:0008104 IBA
    neuron projection development GO:0031175 IMP
    regulation of protein localization GO:0032880 IEA
    regulation of protein localization GO:0032880 ISO
    cell division GO:0051301 IEA
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IEA
    cilium assembly GO:0060271 ISO
    cilium assembly GO:0060271 ISS
    cytoskeleton-dependent cytokinesis GO:0061640 IBA
Subcellular Localization
    exocyst GO:0000145 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    spindle GO:0005819 IEA
    plasma membrane GO:0005886 ISO
    axoneme GO:0005930 IEA
    axoneme GO:0005930 ISO
    septin ring GO:0005940 IBA
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    actin cytoskeleton GO:0015629 IEA
    actin cytoskeleton GO:0015629 ISO
    microtubule cytoskeleton GO:0015630 IBA
    midbody GO:0030496 IEA
    septin complex GO:0031105 IBA
    septin complex GO:0031105 IEA
    septin complex GO:0031105 ISO
    cell division site GO:0032153 IBA
    cleavage furrow GO:0032154 IEA
    photoreceptor connecting cilium GO:0032391 IEA
    photoreceptor connecting cilium GO:0032391 ISO
    ciliary transition zone GO:0035869 ISO
    cell projection GO:0042995 ISO
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    synapse GO:0045202 IEA
    synapse GO:0045202 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    ciliary membrane GO:0060170 IEA
    ciliary membrane GO:0060170 ISO
    ciliary membrane GO:0060170 ISS
    sperm annulus GO:0097227 IEA
    sperm annulus GO:0097227 ISO
    non-motile cilium GO:0097730 ISO
    presynapse GO:0098793 IDA
 Experiment description of studies that identified Sept2 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Sept2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mib1  
Affinity Capture-MS Rattus norvegicus
2 Park7 117287
Affinity Capture-MS Rattus norvegicus
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