Gene description for Hnrnpk
Gene name heterogeneous nuclear ribonucleoprotein K
Gene symbol Hnrnpk
Other names/aliases Csbp
Hnrpk
Species Rattus norvegicus
 Database cross references - Hnrnpk
ExoCarta ExoCarta_117282
Vesiclepedia VP_117282
Entrez Gene 117282
UniProt P61980  
 Hnrnpk identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hnrnpk
Molecular Function
    cis-regulatory region sequence-specific DNA binding GO:0000987 IDA
    DNA binding GO:0003677 IEA
    double-stranded DNA binding GO:0003690 IDA
    single-stranded DNA binding GO:0003697 IDA
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 IBA
    mRNA 3'-UTR binding GO:0003730 IDA
    protein binding GO:0005515 IPI
    lamin binding GO:0005521 IPI
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 IPI
    protein domain specific binding GO:0019904 ISO
    pre-mRNA 3'-splice site binding GO:0030628 IDA
    heat shock protein binding GO:0031072 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    actinin binding GO:0042805 IPI
    ribonucleoprotein complex binding GO:0043021 IDA
    ribonucleoprotein complex binding GO:0043021 IPI
    ATPase binding GO:0051117 IPI
    mRNA CDS binding GO:1990715 IDA
    C-rich single-stranded DNA binding GO:1990829 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    ovarian follicle development GO:0001541 IMP
    kidney development GO:0001822 IEP
    liver development GO:0001889 IEP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    mRNA processing GO:0006397 IEA
    acute-phase response GO:0006953 IEP
    peripheral nervous system development GO:0007422 IEP
    RNA splicing GO:0008380 IEA
    male gonad development GO:0008584 IEP
    negative regulation of gene expression GO:0010629 IMP
    positive regulation of neuron projection development GO:0010976 IMP
    regulation of low-density lipoprotein particle clearance GO:0010988 IEA
    regulation of low-density lipoprotein particle clearance GO:0010988 ISO
    response to activity GO:0014823 IEP
    cerebellum development GO:0021549 IEP
    hippocampus development GO:0021766 IEP
    cerebral cortex development GO:0021987 IDA
    cerebral cortex development GO:0021987 IEP
    lung development GO:0030324 IEP
    regulatory ncRNA-mediated heterochromatin formation GO:0031048 IEA
    regulatory ncRNA-mediated heterochromatin formation GO:0031048 ISO
    positive regulation of myelination GO:0031643 IMP
    cellular response to insulin stimulus GO:0032869 IDA
    cellular response to insulin stimulus GO:0032869 IEP
    positive regulation of RNA splicing GO:0033120 IMP
    camera-type eye development GO:0043010 IEP
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of DNA-templated transcription GO:0045892 IEA
    negative regulation of DNA-templated transcription GO:0045892 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    regulation of mRNA splicing, via spliceosome GO:0048024 IBA
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISO
    positive regulation of receptor-mediated endocytosis GO:0048260 IEA
    positive regulation of receptor-mediated endocytosis GO:0048260 ISO
    thymus development GO:0048538 IEP
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    positive regulation of synaptic transmission GO:0050806 IMP
    random inactivation of X chromosome GO:0060816 IEA
    random inactivation of X chromosome GO:0060816 ISO
    positive regulation of dendritic spine development GO:0060999 IMP
    cellular response to amino acid stimulus GO:0071230 IEP
    cellular response to lead ion GO:0071284 IEP
    cellular response to glucose stimulus GO:0071333 IEP
    cellular response to rapamycin GO:0072752 IEP
    positive regulation of synapse maturation GO:0090129 IMP
    regulation of postsynapse organization GO:0099175 IDA
    regulation of postsynapse organization GO:0099175 IMP
    positive regulation of long-term synaptic potentiation GO:1900273 IMP
    response to salt GO:1902074 IEP
    regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902165 ISO
    positive regulation of dendrite extension GO:1903861 IMP
    cellular response to forskolin GO:1904322 IDA
    positive regulation of low-density lipoprotein receptor activity GO:1905599 ISO
    positive regulation of protein localization to cell surface GO:2000010 IMP
    negative regulation of branching morphogenesis of a nerve GO:2000173 IMP
Subcellular Localization
    chromatin GO:0000785 IEA
    chromatin GO:0000785 ISO
    chromatin GO:0000785 ISS
    podosome GO:0002102 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cell cortex GO:0005938 IDA
    cytoplasmic stress granule GO:0010494 ISO
    protein-DNA complex GO:0032993 IDA
    cell projection GO:0042995 IEA
    dendritic spine GO:0043197 IDA
    axon terminus GO:0043679 IDA
    anchoring junction GO:0070161 IEA
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
    postsynapse GO:0098794 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Hnrnpk in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hnrnpk
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
2 Park7 117287
Affinity Capture-MS Rattus norvegicus
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