Gene description for Lman1
Gene name lectin, mannose-binding, 1
Gene symbol Lman1
Other names/aliases p58
Species Rattus norvegicus
 Database cross references - Lman1
ExoCarta ExoCarta_116666
Vesiclepedia VP_116666
Entrez Gene 116666
UniProt Q62902  
 Lman1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Lman1
Molecular Function
    calcium ion binding GO:0005509 TAS
    D-mannose binding GO:0005537 IBA
    D-mannose binding GO:0005537 TAS
    carbohydrate binding GO:0030246 IEA
    identical protein binding GO:0042802 IDA
    metal ion binding GO:0046872 ISO
Biological Process
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 TAS
    endoplasmic reticulum organization GO:0007029 IEA
    endoplasmic reticulum organization GO:0007029 ISO
    Golgi organization GO:0007030 IEA
    Golgi organization GO:0007030 ISO
    positive regulation of organelle organization GO:0010638 IEA
    positive regulation of organelle organization GO:0010638 ISO
    protein exit from endoplasmic reticulum GO:0032527 ISO
    early endosome to Golgi transport GO:0034498 ISO
    negative regulation of protein targeting to mitochondrion GO:1903215 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IBA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IDA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 TAS
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    membrane GO:0016020 IEA
    sarcomere GO:0030017 IEA
    sarcomere GO:0030017 ISO
    COPII-coated ER to Golgi transport vesicle GO:0030134 IBA
    COPII-coated ER to Golgi transport vesicle GO:0030134 IEA
    COPII-coated ER to Golgi transport vesicle GO:0030134 ISO
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 IEA
    glutamatergic synapse GO:0098978 IDA
    postsynaptic Golgi apparatus GO:0150051 IDA
 Experiment description of studies that identified Lman1 in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Lman1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmed9 361207
Co-fractionation Rattus norvegicus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here